Type

Journal Article

Authors

K C Conlon
P F Ridgway
P Crotty
N Swan
A McDermott
J F Murphy
M Clynes
C Clarke
P Doolan
M J Smith
and 1 others

Subjects

Biochemistry

Topics
oligonucleotide array sequence analysis cell line tumor drug therapy aged plaur protein human cell survival male receptors urokinase plasminogen activator pancreatic neoplasms pathology pharmacology middle aged adenocarcinoma neoplasm invasiveness tetradecanoylphorbol acetate tumor markers biological gene expression profiling secondary drug effects aged 80 and over real time polymerase chain reaction matrix metalloproteinase 1 receptors interleukin 1 metabolism humans adolescent gene expression female genetics

Invasive markers identified by gene expression profiling in pancreatic cancer. (2011)

Abstract Molecular profiling has proven utility as a diagnostic and predictive tool in clinical oncology. However, a clinically relevant gene expression profile in pancreatic cancer remains elusive. Primary and metastatic pancreatic cancer cell lines (BxPC-3 and AsPC-1), were stimulated with phorbol-12-myristate 13-acetate (PMA), a known inducer of cell invasion. Affymetrix gene expression microarray analysis was performed, comparing gene expression to unstimulated controls. Differential expression was identified using ArrayAssist, and confirmed using quantitative real-time PCR. Bioinformatic analysis was performed using Pathway Studio and GOstat. The derived gene expression was further validated in fresh frozen pancreatic tumour samples. The ability of the derived 3 gene expression markersto differentiate between pancreatic adenocarcinoma (PDAC) and other neoplasms, and its association with clinicopathological variables was examined. PMA-induced significant changes in cell line gene expression, from which distinctive 3 potential invasive markers were derived. Expression of these genes, uPA, MMP-1 and IL1-R1 was confirmed in human pancreatic tumours, and was found to differentiate PDAC from other pancreatic neoplasms. The expression of IL1-R1 in PDAC is a novel finding. We found that the expression of MMP-1 was associated with high-grade PDAC (p = 0.035, Wilcoxon rank sum). We have identified three potential invasive markers, uPA, MMP-1 and IL1-R1, whose gene expression may differentiate PDAC from other pancreatic neoplasms, and potentially reflect a more invasive phenotype.
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Full list of authors on original publication

K C Conlon, P F Ridgway, P Crotty, N Swan, A McDermott, J F Murphy, M Clynes, C Clarke, P Doolan, M J Smith and 1 others

Experts in our system

1
K C Conlon
IT Tallaght
Total Publications: 57
 
2
P F Ridgway
IT Tallaght
Total Publications: 41
 
3
P Crotty
IT Tallaght
Total Publications: 22
 
4
N Swan
IT Tallaght
Total Publications: 19
 
5
Martin Clynes
Dublin City University
Total Publications: 209
 
6
Colin Clarke
Dublin City University
Total Publications: 26
 
7
Padraig Doolan
Dublin City University
Total Publications: 37