Type

Journal Article

Authors

Dearbháile Morris
Bernie Austin
James R Johnson
Akke Vellinga
Martin Cormican
Catherine Ludden

Subjects

Microbiology

Topics
staphylococcus aureus long term care colonisation vancomycin antimicrobial prescribing enterobacteriaceae e coli beta lactamase

Colonisation with ESBL-producing and carbapenemase-producing Enterobacteriaceae, vancomycin-resistant enterococci, and meticillin-resistant Staphylococcus aureus in a long-term care facility over one year. (2015)

Abstract This study examined colonisation with and characteristics of antimicrobial-resistant organisms among residents of a long-term care facility (LTCF) over one year, including strain persistence and molecular diversity among isolates of extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae. Sixty-four residents of a LTCF were recruited (51 at baseline, 13 during the year). Data on dependency levels, hospitalisations, and antimicrobial prescribing were collected. Nasal and rectal swabs and catheter urine specimens were examined quarterly, using chromogenic agars, for ESBL-producing Enterobacteriaceae, carbapenemase-producing Enterobacteriaceae (CPE), vancomycin-resistant enterococci (VRE), and meticillin-resistant S. aureus (MRSA). All ESBL-producing E. coli (ESBL-EC) were characterised by pulsed-field gel electrophoresis (PFGE) and PCR to assess for sequence type (ST) ST131, its resistance-associated H30 and H30-Rx subclones, and bla CTX-M, bla TEM, bla SHV, and bla OXA-1. The overall number of residents colonised, by organism, was as follows: ESBL-EC, 35 (55%); MRSA, 17 (27%); ESBL-producing K. pneumoniae (ESBL-KP), 5 (8%); VRE, 2 (3%) and CPE, 0 (0%). All 98 ESBL-EC isolates were H30-Rx ST131, with bla CTX-M-group 1. By PFGE, a group of 91 ESBL-EC (from 33 participants) had ≥ 85% similar profiles and resembled UK epidemic strain A/ international pulsotype PFGE812. Sequential ESBL-EC from individual residents were closely related. Six ESBL-KP isolates, from five participants, had bla CTX-M-group 1 and by PFGE were closely related. Colonisation with ESBL and MRSA was associated with location within the LTCF and previous exposure to antimicrobials. Among LTCF residents, colonisation with ESBL-EC and MRSA was common. All ESBL-EC were H30-Rx ST131, consistent with clonal dissemination.
Collections Ireland -> National University of Ireland Galway -> PubMed

Full list of authors on original publication

Dearbháile Morris, Bernie Austin, James R Johnson, Akke Vellinga, Martin Cormican, Catherine Ludden

Experts in our system

1
B Austin
National University of Ireland Galway
Total Publications: 3
 
2
Akke Vellinga
National University of Ireland Galway
Total Publications: 21
 
3
Martin Cormican
National University of Ireland Galway
Total Publications: 51
 
4
C Ludden
National University of Ireland Galway
Total Publications: 4