Type

Journal Article

Authors

K. Kavanagh
N G McElvaney
E P Reeves
D Bergin
N O'Reilly

Subjects

Microbiology

Topics
bacillus cell movement bacterial proteins cap18 lipopolysaccharide binding protein mite infestations enzyme linked immunosorbent assay rosacea immunology cell migration assays leukocyte animals electrophoresis polyacrylamide gel metabolism biosynthesis antigens bacterial antimicrobial cationic peptides drug effects neutrophils matrix metalloproteinase 9 cytokines pharmacology cell degranulation mites humans neutrophil activation

Demodex-associated bacterial proteins induce neutrophil activation. (2011)

Abstract Patients with rosacea demonstrate a higher density of Demodex mites in their skin than do controls. A bacterium isolated from a Demodex mite from a patient with papulopustular rosacea (PPR) was previously shown to provoke an immune response in patients with PPR or ocular rosacea, thus suggesting a possible role for bacterial proteins in the aetiology of this condition. To examine the response of neutrophils to proteins derived from a bacterium isolated from a Demodex mite. Bacterial cells were lysed and proteins were partially purified by ÄKTA fast protein liquid chromatography. Isolated neutrophils were exposed to bacterial proteins and monitored for alterations in migration, degranulation and cytokine production. Neutrophils exposed to proteins from Bacillus cells demonstrated increased levels of migration and elevated release of matrix metalloprotease 9, an enzyme known to degrade collagen, and cathelicidin, an antimicrobial peptide. In addition, neutrophils exposed to the bacterial proteins demonstrated elevated rates of interleukin 8 and tumour necrosis factor-α production. Proteins produced by a bacterium isolated from a Demodex mite have the ability to increase the migration, degranulation and cytokine production abilities of neutrophils. These results suggest that bacteria may play a role in the inflammatory erythema associated with rosacea.
Collections Ireland -> Maynooth University -> PubMed

Full list of authors on original publication

K. Kavanagh, N G McElvaney, E P Reeves, D Bergin, N O'Reilly

Experts in our system

1
Kevin Kavanagh
Maynooth University
Total Publications: 183
 
2
Noel G McElvaney
Royal College of Surgeons in Ireland
Total Publications: 194
 
3
Emer P Reeves
Royal College of Surgeons in Ireland
Total Publications: 63
 
4
David A Bergin
Royal College of Surgeons in Ireland
Total Publications: 28