Journal Article


Kevin Kavanagh
Martin Clynes
Joanne Keenan
Lisa Murphy
David Bergin



larva insect proteins animals glucans rna messenger microbiology saccharomyces cerevisiae spectrometry proteome mass defensins polysaccharides reverse transcriptase polymerase chain reaction matrix assisted laser gallerimycin moths transferrin gene expression regulation immunology laminaran chemistry genes insect genetics survival analysis hemolymph candida albicans electrophoresis gel two dimensional antimicrobial cationic peptides

Pre-exposure to yeast protects larvae of Galleria mellonella from a subsequent lethal infection by Candida albicans and is mediated by the increased expression of antimicrobial peptides. (2005)

Abstract Pre-exposure of the larvae of Galleria mellonella to Candida albicans or Saccharomyces cerevisiae protects against a subsequent infection with 10(6) C. albicans cells. This protection can also be induced by exposing larvae to glucan or laminarin prior to the administration of the potentially lethal inoculum. Analysis of the genes coding for galiomicin, a defensin in G. mellonella, a cysteine-rich antifungal peptide gallerimycin, an iron-binding protein transferrin and an inducible metalloproteinase inhibitor (IMPI) from G. mellonella demonstrated increased expression, which is at its highest after 24 h of the initial inoculum. Examination of the expression of proteins in the insect haemolymph using 2D electrophoresis and MALDI TOF analysis revealed an increased expression of a number of proteins associated with the insect immune response to infection 24 h after the initial exposure. This study demonstrates that the larvae of G. mellonella can withstand a lethal inoculum of C. albicans if pre-exposed to a non-lethal dose of yeast or polysaccharide 24 h previously which is mediated by increased expression of a number of antimicrobial peptides and the appearance of a number of peptides in the challenged larvae.
Collections Ireland -> Maynooth University -> PubMed

Full list of authors on original publication

Kevin Kavanagh, Martin Clynes, Joanne Keenan, Lisa Murphy, David Bergin

Experts in our system

Kevin Kavanagh
Maynooth University
Total Publications: 183
Martin Clynes
Dublin City University
Total Publications: 232
Joanne Keenan
Dublin City University
Total Publications: 27
Lisa Murphy
University College Dublin
David A Bergin
Royal College of Surgeons in Ireland
Total Publications: 28