Journal Article


Sean Doyle
Kevin Kavanagh
Olaf Scheibner
Claire Neville
Kathrin Reiber
Emer P. Reeves



genes fungal oxidative stress chemistry time factors fungal proteins aspergillus fumigatus mass base sequence enzymology cloning molecular spectrometry phenotype matrix assisted laser genome fungal reverse transcriptase polymerase chain reaction peptide synthases molecular sequence data pes1 protein aspergillus fumigatus physiology

A nonribosomal peptide synthetase (Pes1) confers protection against oxidative stress in Aspergillus fumigatus. (2006)

Abstract Aspergillus fumigatus is an important human fungal pathogen. The Aspergillus fumigatus genome contains 14 nonribosomal peptide synthetase genes, potentially responsible for generating metabolites that contribute to organismal virulence. Differential expression of the nonribosomal peptide synthetase gene, pes1, in four strains of Aspergillus fumigatus was observed. The pattern of pes1 expression differed from that of a putative siderophore synthetase gene, sidD, and so is unlikely to be involved in iron acquisition. The Pes1 protein (expected molecular mass 698 kDa) was partially purified and identified by immunoreactivity, peptide mass fingerprinting (36% sequence coverage) and MALDI LIFT-TOF/TOF MS (four internal peptides sequenced). A pes1 disruption mutant (delta pes1) of Aspergillus fumigatus strain 293.1 was generated and confirmed by Southern and western analysis, in addition to RT-PCR. The delta pes1 mutant also showed significantly reduced virulence in the Galleria mellonella model system (P < 0.001) and increased sensitivity to oxidative stress (P = 0.002) in culture and during neutrophil-mediated phagocytosis. In addition, the mutant exhibited altered conidial surface morphology and hydrophilicity, compared to Aspergillus fumigatus 293.1. It is concluded that pes1 contributes to improved fungal tolerance against oxidative stress, mediated by the conidial phenotype, during the infection process.
Collections Ireland -> Maynooth University -> PubMed

Full list of authors on original publication

Sean Doyle, Kevin Kavanagh, Olaf Scheibner, Claire Neville, Kathrin Reiber, Emer P. Reeves

Experts in our system

Sean Doyle
Maynooth University
Total Publications: 130
Kevin Kavanagh
Maynooth University
Total Publications: 183
Emer P Reeves
Royal College of Surgeons in Ireland
Total Publications: 63