Type

Journal Article

Authors

Catherine M Greene
Marcus A Mall
Noel G McElvaney
Paul McNally
Gerard Higgins
Kevin Molloy
Kevin Gaughan
Raman Agrawal
Sebastian F Vencken
Irene K Oglesby

Subjects

Microbiology

Topics
interleukin 8 anti inflammatory mir 17 interleukin 17 medicine and health sciences cystic fibrosis transmembrane conductance regulator microrna cystic fibrosis cell lines messenger rna epithelial cells unclassified drug bronchoalveolar lavage fluid

miR-17 overexpression in cystic fibrosis airway epithelial cells decreases interleukin-8 production. (2015)

Abstract Interleukin (IL)-8 levels are higher than normal in cystic fibrosis (CF) airways, causing neutrophil infiltration and non-resolving inflammation. Overexpression of microRNAs that target IL-8 expression in airway epithelial cells may represent a therapeutic strategy for cystic fibrosis.IL-8 protein and mRNA were measured in cystic fibrosis and non-cystic fibrosis bronchoalveolar lavage fluid and bronchial brushings (n=20 per group). miRNAs decreased in the cystic fibrosis lung and predicted to target IL-8 mRNA were quantified in ╬▓ENaC-transgenic, cystic fibrosis transmembrane conductance regulator (Cftr)-/- and wild-type mice, primary cystic fibrosis and non-cystic fibrosis bronchial epithelial cells and a range of cystic fibrosis versus non-cystic fibrosis airway epithelial cell lines or cells stimulated with lipopolysaccharide, Pseudomonas-conditioned medium or cystic fibrosis bronchoalveolar lavage fluid. The effect of miRNA overexpression on IL-8 protein production was measured.miR-17 regulates IL-8 and its expression was decreased in adult cystic fibrosis bronchial brushings, ╬▓ENaC-transgenic mice and bronchial epithelial cells chronically stimulated with Pseudomonas-conditioned medium. Overexpression of miR-17 inhibited basal and agonist-induced IL-8 protein production in F508del-CFTR homozygous CFTE29o(-) tracheal, CFBE41o(-) and/or IB3 bronchial epithelial cells.These results implicate defective CFTR, inflammation, neutrophilia and mucus overproduction in regulation of miR-17. Modulating miR-17 expression in cystic fibrosis bronchial epithelial cells may be a novel anti-inflammatory strategy for cystic fibrosis and other chronic inflammatory airway diseases.
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Full list of authors on original publication

Catherine M Greene, Marcus A Mall, Noel G McElvaney, Paul McNally, Gerard Higgins, Kevin Molloy, Kevin Gaughan, Raman Agrawal, Sebastian F Vencken, Irene K Oglesby

Experts in our system

1
Catherine M Greene
Royal College of Surgeons in Ireland
Total Publications: 150
 
2
Marcus A Mall
Royal College of Surgeons in Ireland
Total Publications: 4
 
3
Noel G McElvaney
Royal College of Surgeons in Ireland
Total Publications: 194
 
4
Paul McNally
Royal College of Surgeons in Ireland
Total Publications: 26
 
5
Gerard Higgins
Royal College of Surgeons in Ireland
Total Publications: 8
 
6
Kevin Molloy
Royal College of Surgeons in Ireland
Total Publications: 19
 
7
Sebastian Vencken
Royal College of Surgeons in Ireland
Total Publications: 13