Type

Journal Article

Authors

Maria M Byrne
Jochen H M Prehn
Caoimhín Concannon
Marie Burke
Ailbhe McCarthy
Ross Gallagher
Shona Pfeiffer
Jasmin Schmid
Britta Engelbrecht
Siobhan Bacon

Subjects

Veterinary

Topics
beta cell gene expression microrna mirna diabetes mellitus type 2 insulin secretion urine hnf1a mody physics urinary biomarker diabetes mellitus type 1 mir 103 diabetes associated icrorna mir 224 diabetes mellitus physiology

MicroRNA-224 is Readily Detectable in Urine of Individuals with Diabetes Mellitus and is a Potential Indicator of Beta-Cell Demise. (2015)

Abstract MicroRNA (miRNA) are a class of non-coding, 19-25 nucleotide RNA critical for network-level regulation of gene expression. miRNA serve as paracrine signaling molecules. Using an unbiased array approach, we previously identified elevated levels of miR-224 and miR-103 to be associated with a monogenic form of diabetes; HNF1A-MODY. miR-224 is a novel miRNA in the field of diabetes. We sought to explore the role of miR-224 as a potential biomarker in diabetes, and whether such diabetes-associated-miRNA can also be detected in the urine of patients. Absolute levels of miR-224 and miR-103 were determined in the urine of n = 144 individuals including carriers of a HNF1A mutation, participants with type 1 diabetes mellitus (T1DM), type 2 diabetes mellitus (T2DM) and normal controls. Expression levels were correlated with clinical and biochemical parameters. miR-224 was significantly elevated in the urine of carriers of a HNF1A mutation and participants with T1DM. miR-103 was highly expressed in urine across all diabetes cohorts when compared to controls. For both miR-224 and-103, we found a significant correlation between serum and urine levels (p < 0.01). We demonstrate that miRNA can be readily detected in the urine independent of clinical indices of renal dysfunction. We surmise that the differential expression levels of miR-224 in both HNF1A-MODY mutation carriers and T1DM may be an attempt to compensate for beta-cell demise.
Collections Ireland -> Royal College of Surgeons in Ireland -> PubMed
Ireland -> Royal College of Surgeons in Ireland -> Physiology and Medical Physics Articles
Ireland -> Royal College of Surgeons in Ireland -> Department of Physiology and Medical Physics

Full list of authors on original publication

Maria M Byrne, Jochen H M Prehn, Caoimhín Concannon, Marie Burke, Ailbhe McCarthy, Ross Gallagher, Shona Pfeiffer, Jasmin Schmid, Britta Engelbrecht, Siobhan Bacon

Experts in our system

1
Jochen H M Prehn
Royal College of Surgeons in Ireland
Total Publications: 206
 
2
Caoimhín G Concannon
Royal College of Surgeons in Ireland
Total Publications: 46
 
3
Shona Pfeiffer
Royal College of Surgeons in Ireland
Total Publications: 14
 
4
Jasmin Schmid
Royal College of Surgeons in Ireland
Total Publications: 13