Type

Journal Article

Authors

Dmitri B Papkovsky
Ingo Klimant
Vladimir P Baklaushev
Jochen Prehn
Bernhard J Müller
Shiwen Sun
Heiko Düssmann
Sergey M Borisov
Ruslan I Dmitriev

Subjects

Chemistry

Topics
live cell imaging conjugated polymer low toxicity cells surface properties high resolution 3d imaging cationic nanoparticles

Versatile Conjugated Polymer Nanoparticles for High-Resolution O2 Imaging in Cells and 3D Tissue Models. (2015)

Abstract High brightness, chemical and photostability, tunable characteristics, and spectral and surface properties are important attributes for nanoparticle probes designed for live cell imaging. We describe a class of nanoparticles for high-resolution imaging of O2 that consists of a substituted conjugated polymer (polyfluorene or poly(fluorene-alt-benzothiadiazole)) acting as a FRET antenna and a fluorescent reference with covalently bound phosphorescent metalloporphyrin (PtTFPP, PtTPTBPF). The nanoparticles prepared from such copolymers by precipitation method display stability, enhanced (>5-10 times) brightness under one- and two-photon excitation, compatibility with ratiometric and lifetime-based imaging modes, and low toxicity for cells. Their cell-staining properties can be modulated with positively and negatively charged groups grafted to the backbone. The "zwitter-ionic" nanoparticles show high cell-staining efficiency, while their cell entry mechanisms differ for the different 3D models. When injected in the bloodstream, the cationic and anionic nanoparticles show similar distribution in vivo. These features and tunable properties make the conjugated polymer based phosphorescent nanoparticles a versatile tool for quantitative O2 imaging with a broad range of cell and 3D tissue models.
Collections Ireland -> Royal College of Surgeons in Ireland -> PubMed

Full list of authors on original publication

Dmitri B Papkovsky, Ingo Klimant, Vladimir P Baklaushev, Jochen Prehn, Bernhard J Müller, Shiwen Sun, Heiko Düssmann, Sergey M Borisov, Ruslan I Dmitriev

Experts in our system

1
Dmitri B Papkovsky
University College Cork
Total Publications: 89
 
2
Jochen H M Prehn
Royal College of Surgeons in Ireland
Total Publications: 206
 
3
Heiko Düssmann
Royal College of Surgeons in Ireland
Total Publications: 45
 
4
Ruslan I Dmitriev
University College Cork
Total Publications: 41