Journal Article


Eleanor J Molloy
Anne Twomey
John Murphy
Paul Downey
Eoghan Mooney
Veronica Donoghue
Chike Onwuneme
Tim Grant
Amanda O'Neill
William Watson
and 1 others



immune response outcomes toll like receptor 4 oxygen flow cytometry brain injury infants neutrophil activation

Neutrophil and monocyte Toll-Like Receptor 4, CD11b and Reactive Oxygen Intermediates and Neuroimaging Outcomes in Preterm Infants. (2014)

Abstract Activated leukocytes and infection are implicated in neonatal brain injury. Leukocyte surface receptors are increased in stroke models and may be targets for future adjunctive therapies. Serial blood samples were analysed from preterm infants (n= 51; <32 weeks gestation) on day 0, 1, 2 and 7 of life. Monocyte and neutrophil activation were evaluated via flow cytometry at baseline and following endotoxin stimulation ex vivo by measuring CD11b (activation), Toll-Like Receptor 4 (TLR-4; endotoxin recognition) expression and intracellular reactive oxygen intermediate (ROI) production (function). Control Preterm infants with normal neuroimaging had elevated baseline CD11b and TLR-4 expression, and ROI production compared with adults as well as a robust immune response following endotoxin stimulation. Preterm infants with abnormal neuroimaging had increased neutrophil TLR-4 and ROI compared with all controls. Preterm infants have a robust immune response compared to adults. Increased TLR-4 expression in preterm infants with abnormal neuroimaging is similar to findings in adult stroke. In addition, ROI production may cause tissue injury. The modulation of these responses may be beneficial in preterm inflammatory disorders.Pediatric Research (2015); doi:10.1038/pr.2015.66.
Collections Ireland -> Royal College of Surgeons in Ireland -> PubMed

Full list of authors on original publication

Eleanor J Molloy, Anne Twomey, John Murphy, Paul Downey, Eoghan Mooney, Veronica Donoghue, Chike Onwuneme, Tim Grant, Amanda O'Neill, William Watson and 1 others

Experts in our system

Eleanor J Molloy
TU Dublin (Tallaght Campus)
Total Publications: 22
Chike Onwuneme
TU Dublin (Tallaght Campus)
Total Publications: 6
Amanda J O'Neill
University College Dublin
Total Publications: 46
William R Watson
Trinity College Dublin