Type

Journal Article

Authors

Fergal J O'Brien
Sally-Ann Cryan
Alan Hibbitts
Emmet M Thompson
Adolfo López-Noriega
Elaine Quinlan

Subjects

Pharmacology

Topics
bone growth tissue engineering collagen scaffolds controlled growth endothelial cells bone regeneration spray drying vascular endothelial growth factor

Controlled release of vascular endothelial growth factor from spray-dried alginate microparticles in collagen-hydroxyapatite scaffolds for promoting vascularization and bone repair. (2014)

Abstract A major limitation with current tissue-engineering approaches is creating functionally vascularized constructs that can successfully integrate with the host; this often leads to implant failure, due to avascular necrosis. In order to overcome this, the objective of the present work was to develop a method to incorporate growth factor-eluting alginate microparticles (MPs) into freeze-dried, collagen-based scaffolds. A collagen-hydroxyapatite (CHA) scaffold, previously optimized for bone regeneration, was functionalized for the sustained delivery of an angiogenic growth factor, vascular endothelial growth factor (VEGF), with the aim of facilitating angiogenesis and enhancing bone regeneration. VEGF was initially encapsulated in alginate MPs by spray-drying, producing particles of < 10 µm in diameter. This process was found to effectively encapsulate and control VEGF release while maintaining its stability and bioactivity post-processing. These VEGF-MPs were then incorporated into CHA scaffolds, leading to homogeneous distribution throughout the interconnected scaffold pore structure. The scaffolds were capable of sustained release of bioactive VEGF for up to 35 days, which was proficient at increasing tubule formation by endothelial cells in vitro. When implanted in vivo in a rat calvarial defect model, this scaffold enhanced vessel formation, resulting in increased bone regeneration compared to empty-defect and VEGF-free scaffolds. This biologically functionalized scaffold, composed entirely of natural-based materials, may offer an ideal platform to promote angiogenesis and tissue regeneration. Copyright © 2015 John Wiley & Sons, Ltd.
Collections Ireland -> Royal College of Surgeons in Ireland -> PubMed

Full list of authors on original publication

Fergal J O'Brien, Sally-Ann Cryan, Alan Hibbitts, Emmet M Thompson, Adolfo López-Noriega, Elaine Quinlan

Experts in our system

1
Fergal J O'Brien
Royal College of Surgeons in Ireland
Total Publications: 268
 
2
Sally-Ann Cryan
Royal College of Surgeons in Ireland
Total Publications: 67
 
3
Alan Hibbitts
Royal College of Surgeons in Ireland
Total Publications: 9
 
4
Emmet M Thompson
Royal College of Surgeons in Ireland
Total Publications: 17
 
5
Adolfo López-Noriega
Royal College of Surgeons in Ireland
Total Publications: 10
 
6
Elaine Quinlan
Royal College of Surgeons in Ireland
Total Publications: 11