Type

Journal Article

Authors

S W Kerrigan
F J O'Brien
T J Foster
N Kavanagh
T Claro

Subjects

Microbiology

Topics
protein staphylococcus epidermidis bone loss serine lactococcus lactis cell wall v v alpha

Staphylococcus epidermidis serine-aspartate repeat protein G (SdrG) binds to osteoblast integrin alpha V beta 3. (2014)

Abstract Staphylococcus epidermidis is the leading etiologic agent of orthopaedic implant infection. Contamination of the implanted device during insertion allows bacteria gain entry into the sterile bone environment leading to condition known as osteomyelitis. Osteomyelitis is characterised by weakened bones associated with progressive bone loss. The mechanism through which S. epidermidis interacts with bone cells to cause osteomyelitis is poorly understood. We demonstrate here that S. epidermidis can bind to osteoblasts in the absence of matrix proteins. S. epidermidis strains lacking the cell wall protein SdrG had a significantly reduced ability to bind to osteoblasts. Consistent with this, expression of SdrG in Lactococcus lactis resulted in significantly increased binding to the osteoblasts. Protein analysis identified that SdrG contains a potential integrin recognition motif. αVβ3 is a major integrin expressed on osteoblasts and typically recognises RGD motifs in its ligands. Our results demonstrate that S. epidermidis binds to recombinant purified αVβ3, and that a mutant lacking SdrG failed to bind. Blocking αVβ3 on osteoblasts significantly reduced binding to S. epidermidis. These studies are the first to identify a mechanism through which S. epidermidis binds to osteoblasts and potentially offers a mechanism through which implant infection caused by S. epidermidis leads to osteomyelitis.
Collections Ireland -> Royal College of Surgeons in Ireland -> PubMed

Full list of authors on original publication

S W Kerrigan, F J O'Brien, T J Foster, N Kavanagh, T Claro

Experts in our system

1
Steven W Kerrigan
Royal College of Surgeons in Ireland
Total Publications: 37
 
2
Fergal J O'Brien
Royal College of Surgeons in Ireland
Total Publications: 268
 
3
Timothy Foster
Trinity College Dublin
Total Publications: 103
 
4
Tania Claro
Dublin City University
Total Publications: 13