Type

Journal Article

Authors

Sally-Ann Cryan
Caroline Jefferies
Joanne M Ramsey
James W Barlow
Colin Burke
Ciaran Lawlor
Ciara Kelly

Subjects

Pharmacology

Topics
drug delivery drug discovery drug carriers high content screening nanoparticles high throughput screening pharmacy and pharmaceutical sciences cell interaction large scale nanotoxicology half life liposomes high content analysis high throughput

High-throughput methods for screening liposome-macrophage cell interaction. (2014)

Abstract Abstract Carriers are often an essential element of drug delivery, bestowing attributes to their cargo such as biocompatibility, enhanced delivery, extended half-life and efficacy as well as mediating specific targeting at a tissue, cell or intracellular level. Liposomes and lipid-based carriers have been investigated for decades for this purpose, many achieving clinical approval including products such as Doxil® and Myocet™. Large-scale compound screens are routinely carried out in the field of drug discovery; however, less work has been done on harnessing high-throughput methods for carrier material screening. Screening the interaction of drug carriers and materials with cells is particularly critical for the development of emerging therapies, including biomedicines, in order to facilitate the development of safe and efficient drug products. Herein, a range of liposomes of neutral, anionic and cationic charge and others that are surface-modified with mannose residues were screened for cell interaction, toxicity and immune reactivity in THP-1-derived macrophages using a high-throughput format. Liposomes were seen to be efficacious in a concentration-dependent and, for mannosylated liposomes, mannosylated cholesterol linker length-dependent manner.
Collections Ireland -> Royal College of Surgeons in Ireland -> PubMed
Ireland -> Royal College of Surgeons in Ireland -> School of Pharmacy
Ireland -> Royal College of Surgeons in Ireland -> School of Pharmacy Articles

Full list of authors on original publication

Sally-Ann Cryan, Caroline Jefferies, Joanne M Ramsey, James W Barlow, Colin Burke, Ciaran Lawlor, Ciara Kelly

Experts in our system

1
Sally-Ann Cryan
Royal College of Surgeons in Ireland
Total Publications: 70
 
2
Caroline A Jefferies
Royal College of Surgeons in Ireland
Total Publications: 50
 
3
Joanne M Ramsey
Royal College of Surgeons in Ireland
Total Publications: 5
 
4
Ciaran Lawlor
Royal College of Surgeons in Ireland
Total Publications: 8