Type

Journal Article

Authors

Catherine M Greene
Stephen G J Smith
Noel G McElvaney
Tidi Hassan
Sebastian Vencken

Subjects

Biochemistry

Topics
microrna mirna microrna prediction messenger rna mrna mir single technology experimental

miR-CATCH: microRNA capture affinity technology. (2014)

Abstract Several experimental methods exist to explore the microRNA (miRNA) regulome. These methods almost exclusively focus on multiple targets bound to a single, or perhaps a few miRNAs of interest. Here, we describe a microRNA capture affinity technology (miR-CATCH) which uses an affinity capture oligonucleotide to co-purify a single target messenger RNA (mRNA) together with all its endogenously bound miRNAs. This bench-top method is similar to RNA immunoprecipitation (RIP) and provides an experimental alternative to computational miRNA target prediction.
Collections Ireland -> Royal College of Surgeons in Ireland -> PubMed

Full list of authors on original publication

Catherine M Greene, Stephen G J Smith, Noel G McElvaney, Tidi Hassan, Sebastian Vencken

Experts in our system

1
Catherine M Greene
Royal College of Surgeons in Ireland
Total Publications: 150
 
2
Stephen Smith
Trinity College Dublin
Total Publications: 27
 
3
Noel G McElvaney
Royal College of Surgeons in Ireland
Total Publications: 194
 
4
Tidi Hassan
Royal College of Surgeons in Ireland
 
5
Sebastian Vencken
Royal College of Surgeons in Ireland
Total Publications: 13