Type

Journal Article

Authors

Jochen H M Prehn
Caoimhín G Concannon
David C Henshall
Eva M Jimenez-Mateos
Mollie R Mitchem
Katie Nolan

Subjects

Biochemistry

Topics
spinal cord superoxide dismutase animals oligoribonucleotides mirn29 microrna mouse micrornas amyotrophic lateral sclerosis genetics metabolism male brain mice pathology mice inbred c57bl drug effects pharmacology superoxide dismutase 1 antagonists inhibitors

Increased expression of microRNA-29a in ALS mice: functional analysis of its inhibition. (2014)

Abstract Endoplasmic reticulum (ER) stress has been implicated in a number of neurodegenerative diseases such as amyotrophic lateral sclerosis (ALS). MicroRNAs are small ribonucleic acids which can modulate protein expression by binding to the 3'UTR of target mRNAs. We recently identified increased miR-29a expression in response to ER stress in neurons, with members of the miR-29 family implicated in cancer and neurodegeneration. We found high expression of miR-29a in the mouse brain and spinal cord by quantitative PCR analysis and increased expression of miR-29a in the spinal cord of SOD1(G93A) transgenic mice, a mouse model of familial ALS. In situ hybridisation experiments revealed increased miR-29a expression in the lumbar spinal cord of SOD1(G93A) transgenic mice from postnatal day 70 onward when compared to wild-type mice. miR-29a knockdown was achieved in the CNS in vivo after a single intracerebroventricular injection of a miR-29a-specific antagomir. While analysis of disease progression and motor function could not identify a significant alteration in ALS disease manifestations, a trend towards increased lifespan was observed in male SOD1(G93A) mice. These findings demonstrate that miR-29a may act as a marker for disease progression in SOD1(G93A) mice, and provide first proof-of-concept for a therapeutic modulation of miR-29a function in ALS.
Collections Ireland -> Royal College of Surgeons in Ireland -> PubMed

Full list of authors on original publication

Jochen H M Prehn, Caoimhín G Concannon, David C Henshall, Eva M Jimenez-Mateos, Mollie R Mitchem, Katie Nolan

Experts in our system

1
Jochen H M Prehn
Royal College of Surgeons in Ireland
Total Publications: 206
 
2
Caoimhín G Concannon
Royal College of Surgeons in Ireland
Total Publications: 46
 
3
David C Henshall
Royal College of Surgeons in Ireland
Total Publications: 127
 
4
Eva M Jimenez-Mateos
Royal College of Surgeons in Ireland
Total Publications: 55
 
5
Katie Nolan
Royal College of Surgeons in Ireland
Total Publications: 5