Type

Journal Article

Authors

B P Kirby
J H M Prehn
H-G König
E C Bunk

Subjects

Biochemistry

Topics
receptors n methyl d aspartate neurogenesis ifenprodil pharmacology cytology nr2b nmda receptor mice inbred c57bl drug effects physiology antagonists inhibitors animals hippocampus mice metabolism cell proliferation piperidines in situ nick end labeling adult stem cells neural stem cells male

Effect of the N-methyl-D-aspartate NR2B subunit antagonist ifenprodil on precursor cell proliferation in the hippocampus. (2013)

Abstract The N-methyl-D-aspartate (NMDA) receptor, one of the ionotropic glutamate receptor, plays important physiological and pathological roles in learning and memory, neuronal development, acute and chronic neurological diseases, and neurogenesis. This work examines the contribution of the NR2B NMDA receptor subunit to adult neurogenesis/cell proliferation under physiological conditions and following an excitotoxic insult. We have previously shown in vitro that a discrete NMDA-induced, excitotoxic injury to the hippocampus results in an increase in neurogenesis within the dentate gyrus. Here we have characterized adult neurogenesis or proliferation, using BrdU, in an in vivo model of excitotoxic injury to the CA1 subfield of the hippocampus. We demonstrate a peak in neural stem cell proliferation/neurogenesis between 6 and 9 days after the excitotoxic insult. Treatment with ifenprodil, an NR2B subunit-specific NMDA receptor antagonist, without prior injury induction, also increased the number of BrdU-positive cells within the DG and posterior periventricle, indicating that ifenprodil itself could modulate the rate of proliferation. Interestingly, though, the increased level of cell proliferation did not change significantly when ifenprodil was administered following an excitotoxic insult. In conclusion, our results suggest and add to the growing evidence that NR2B subunit-containing NMDA receptors play a role in neural stem cell proliferation.
Collections Ireland -> Royal College of Surgeons in Ireland -> PubMed

Full list of authors on original publication

B P Kirby, J H M Prehn, H-G König, E C Bunk

Experts in our system

1
Brian P Kirby
Royal College of Surgeons in Ireland
Total Publications: 48
 
2
Jochen H M Prehn
Royal College of Surgeons in Ireland
Total Publications: 206
 
3
Hans Georg König
Royal College of Surgeons in Ireland
Total Publications: 22