Type

Journal Article

Authors

David C Henshall
Raymond L Stallings
Sudipto Das
Suzanne F C Miller-Delaney

Subjects

Microbiology

Topics
genetics immunoprecipitation mice status epilepticus promoter regions genetic hydrogen sulfite sulfites dna dna methylation animals disease models animal chemistry oligonucleotide array sequence analysis

Investigating gene promoter methylation in a mouse model of status epilepticus. (2013)

Abstract Epigenetic modification of DNA by methylation of the cytosine present in CG dinucleotides constitutes a key regulatory mechanism in the control of gene expression in neurological diseases. In this chapter, we describe an in-depth methodology of methylated DNA immunoprecipitation used in combination with tiling microarrays (MeDIP-chip) in order to analyze genome-wide gene promoter methylation in the hippocampus of mice following status epilepticus (prolonged seizure). While a specific mouse model and array format are described, the method can be applied to DNA from many tissues to analyze the methylation status of promoter regions across whole genomes, using a wide range of available array formats (both custom designed and commercially catalogued). We conclude the chapter with the description of bisulfite sequencing validation of MeDIP-chip results.
Collections Ireland -> Royal College of Surgeons in Ireland -> PubMed

Full list of authors on original publication

David C Henshall, Raymond L Stallings, Sudipto Das, Suzanne F C Miller-Delaney

Experts in our system

1
David C Henshall
Royal College of Surgeons in Ireland
Total Publications: 127
 
2
Raymond L Stallings
Royal College of Surgeons in Ireland
Total Publications: 59
 
3
Sudipto Das
Royal College of Surgeons in Ireland
Total Publications: 15
 
4
Suzanne F C Miller-Delaney
Royal College of Surgeons in Ireland
Total Publications: 8