Type

Journal Article

Authors

Dermot Kenny
Lourdes Basabe-Desmonts
Sofia Ramström
Antonio J Ricco
David P Foley
Karl Egan
Martin Somers
Bincy Jose
Ana Lopez-Alonso

Subjects

Pharmacology

Topics
cell adhesion flow cytometry cell function microfluidic platforms cardiovascular disease clinical practice blood drug effects

Individual Platelet Adhesion Assay: Measuring Platelet Function and Antiplatelet Therapies in Whole Blood via Digital Quantification of Cell Adhesion. (2013)

Abstract Widespread monitoring of platelet function and the effect of antiplatelet drugs will improve outcomes in cardiovascular patients, but platelet function testing is not routine in clinical practice. We report a rapid, accurate methodology to quantify platelet-protein interactions: a microarray of contact-printed 6-μm fibrinogen dots on a transparent substrate binds platelets from whole blood, one platelet per dot. The fractional occupancy of an array of fibrinogen dots after a predefined incubation time quantitatively assays platelet adhesion to the protein matrix. We demonstrate this technique by measurement of platelet adhesion to fibrinogen as a means to quantify the effect of the P2Y12 and αIIbβ3 receptor inhibitors cangrelor and abciximab, respectively, both in vitro-by incubating the drug with a freshly drawn blood sample-and in blood from patients treated with antiplatelet agents. The effects of single- and dual-antiplatelet therapy are also assessed. Results from this platelet-binding assay are well correlated with standard techniques including flow cytometry and light transmission aggregometry. This assay technology, readily integrated with microfluidic platforms, is generally applicable to the assay of cell-protein interactions and promises more effective, rapid assay of drug effects in cardiovascular disease patients.
Collections Ireland -> Royal College of Surgeons in Ireland -> PubMed

Full list of authors on original publication

Dermot Kenny, Lourdes Basabe-Desmonts, Sofia Ramström, Antonio J Ricco, David P Foley, Karl Egan, Martin Somers, Bincy Jose, Ana Lopez-Alonso

Experts in our system

1
Dermot Kenny
Royal College of Surgeons in Ireland
 
2
Lourdes Basabe-Desmonts
Dublin City University
Total Publications: 15
 
3
Sofia Ramström
Royal College of Surgeons in Ireland
Total Publications: 4
 
4
Antonio J. Ricco
Dublin City University
Total Publications: 24
 
5
Karl Egan
Royal College of Surgeons in Ireland