Type

Journal Article

Authors

David C Henshall
Roger P Simon
Raymond L Stallings
Isabella Bray
Takanori Sano
Eva M Jimenez-Mateos
Ross C McKiernan

Subjects

Biochemistry

Topics
blotting western kainic acid mice inbred c57bl up regulation neurons male cell death pathology animals electroencephalography convulsants gene expression profiling chemically induced mice mirn184 microrna mouse epilepsy real time polymerase chain reaction in situ hybridization metabolism administration dosage reverse transcriptase polymerase chain reaction micrornas genetics seizures hippocampus

Expression profiling the microRNA response to epileptic preconditioning identifies miR-184 as a modulator of seizure-induced neuronal death. (2012)

Abstract Brief seizures (epileptic/seizure preconditioning) are capable of activating endogenous protective pathways in the brain which can temporarily generate a damage-refractory state against subsequent and otherwise harmful episodes of prolonged seizures (tolerance). Altered expression of microRNAs, a class of non-coding RNAs that function post-transcriptionally to regulate mRNA translation has recently been implicated in the molecular mechanism of epileptic tolerance. Here we characterized the effect of seizure preconditioning induced by low-dose systemic kainic acid on microRNA expression in the hippocampus of mice. Seizure preconditioning resulted in up-regulation of 25 mature microRNAs in the CA3 subfield of the mouse hippocampus, with the highest levels detected for miR-184. This finding was supported by real time PCR and in situ hybridization showing increased neuronal miR-184 levels and a reduction in protein levels of a miR-184 target. Inhibiting miR-184 expression in vivo resulted in the emergence of neuronal death after preconditioning seizures and increased seizure-induced neuronal death following status epilepticus in previously preconditioned animals, without altered electrographic seizure durations. The present study suggests miRNA up-regulation after preconditioning may contribute to development of epileptic tolerance and identifies miR-184 as a novel contributor to neuronal survival following both mild and severe seizures.
Collections Ireland -> Royal College of Surgeons in Ireland -> PubMed

Full list of authors on original publication

David C Henshall, Roger P Simon, Raymond L Stallings, Isabella Bray, Takanori Sano, Eva M Jimenez-Mateos, Ross C McKiernan

Experts in our system

1
David C Henshall
Royal College of Surgeons in Ireland
Total Publications: 127
 
2
Raymond L Stallings
Royal College of Surgeons in Ireland
Total Publications: 59
 
3
Isabella Bray
Royal College of Surgeons in Ireland
Total Publications: 35
 
4
Eva M Jimenez-Mateos
Royal College of Surgeons in Ireland
Total Publications: 55