Type

Journal Article

Authors

David C Henshall
Javier deFelipe
Raymond L Stallings
Ronan M Conroy
Donncha F O'Brien
Michael A Farrell
Norman Delanty
Suzanne Prenter
John L Waddington
Colm O'Tuathaigh
and 7 others

Subjects

Biochemistry

Topics
toxicity micrornas prevention control genetics seizures metabolism status epilepticus ca3 region hippocampal up regulation dendritic spines neurons male cell death pathology kainic acid middle aged mice inbred c57bl pyramidal cells mirn134 microrna human gene silencing animals epilepsy temporal lobe mice real time polymerase chain reaction adult drug effects neuroprotective agents humans mirn134 microrna mouse recurrence

Silencing microRNA-134 produces neuroprotective and prolonged seizure-suppressive effects. (2011)

Abstract Temporal lobe epilepsy is a common, chronic neurological disorder characterized by recurrent spontaneous seizures. MicroRNAs (miRNAs) are small, noncoding RNAs that regulate post-transcriptional expression of protein-coding mRNAs, which may have key roles in the pathogenesis of neurological disorders. In experimental models of prolonged, injurious seizures (status epilepticus) and in human epilepsy, we found upregulation of miR-134, a brain-specific, activity-regulated miRNA that has been implicated in the control of dendritic spine morphology. Silencing of miR-134 expression in vivo using antagomirs reduced hippocampal CA3 pyramidal neuron dendrite spine density by 21% and rendered mice refractory to seizures and hippocampal injury caused by status epilepticus. Depletion of miR-134 after status epilepticus in mice reduced the later occurrence of spontaneous seizures by over 90% and mitigated the attendant pathological features of temporal lobe epilepsy. Thus, silencing miR-134 exerts prolonged seizure-suppressant and neuroprotective actions; determining whether these are anticonvulsant effects or are truly antiepileptogenic effects requires additional experimentation.
Collections Ireland -> Royal College of Surgeons in Ireland -> PubMed

Full list of authors on original publication

David C Henshall, Javier deFelipe, Raymond L Stallings, Ronan M Conroy, Donncha F O'Brien, Michael A Farrell, Norman Delanty, Suzanne Prenter, John L Waddington, Colm O'Tuathaigh and 7 others

Experts in our system

1
David C Henshall
Royal College of Surgeons in Ireland
Total Publications: 127
 
2
Raymond L Stallings
Royal College of Surgeons in Ireland
Total Publications: 59
 
3
RonĂ¡n Conroy
Royal College of Surgeons in Ireland
Total Publications: 150
 
4
Donncha F O'Brien
Royal College of Surgeons in Ireland
Total Publications: 19
 
5
Michael A Farrell
Royal College of Surgeons in Ireland
Total Publications: 43
 
6
Norman Delanty
Royal College of Surgeons in Ireland
Total Publications: 49
 
7
John L Waddington
Royal College of Surgeons in Ireland
Total Publications: 170
 
8
Colm M P O'Tuathaigh
University College Cork
Total Publications: 78