Journal Article


Caroline A Jefferies
Jim Johnston
Adrien Kissenpfennig
Kevin Stacey
Barbara Coffey
Jennifer C Byrne
Siobhán Smith
Claire Wynne
Shaun Spence
Joan Ní Gabhann



immunology coculture techniques animals cells cultured biosynthesis deficiency mice tlr4 protein mouse toll like receptor 4 killer cells natural protein tyrosine kinases interferon gamma dendritic cells agammaglobulinaemia tyrosine kinase mice inbred c57bl

Defects in acute responses to TLR4 in Btk-deficient mice result in impaired dendritic cell-induced IFN-γ production by natural killer cells. (2011)

Abstract This study defines a critical role for Btk in regulating TLR4-induced crosstalk between antigen presenting cells (APCs) and natural killer (NK) cells. Reduced levels of IL-12, IL-18 and IFN-γ were observed in Btk-deficient mice and ex vivo generated macrophages and dendritic cells (DCs) following acute LPS administration, whilst enhanced IL-10 production was observed. In addition, upregulation of activation markers and antigen presentation molecules on APCs was also impaired in the absence of Btk. APCs, by virtue of their ability to produce IL-12 and IL-18, are strong inducers of NK-derived IFN-γ. Co-culture experiments demonstrate that Btk-deficient DCs were unable to drive wild-type or Btk-deficient NK cells to induce IFN-γ production, whereas these responses could be restored by exogenous administration of IL-12 and IL-18. Thus Btk is a critical regulator of APC-induced NK cell activation by virtue of its ability to regulate IL-12 and IL-18 production in response to acute LPS administration.
Collections Ireland -> Royal College of Surgeons in Ireland -> PubMed

Full list of authors on original publication

Caroline A Jefferies, Jim Johnston, Adrien Kissenpfennig, Kevin Stacey, Barbara Coffey, Jennifer C Byrne, Siobhán Smith, Claire Wynne, Shaun Spence, Joan Ní Gabhann

Experts in our system

Caroline A Jefferies
Royal College of Surgeons in Ireland
Total Publications: 50
Adrien Kissenpfennig
Dublin Institute of Technology
Total Publications: 11
Siobhán Smith
Royal College of Surgeons in Ireland
Total Publications: 19
Joan Ní Gabhann
Royal College of Surgeons in Ireland
Total Publications: 23