Type

Journal Article

Authors

Fergal J O'Brien
Sally-Ann Cryan
Alan J Hibbitts
Garry P Duffy
Erica G Tierney

Subjects

Biochemistry

Topics
green fluorescent proteins bone regeneration genetics tissue scaffolds transfection mesenchymal stromal cells collagen glycosaminoglycans cell survival luciferases polyethyleneimine administration dosage metabolism plasmids dna animals rats inbred f344 chemistry female rats

The development of non-viral gene-activated matrices for bone regeneration using polyethyleneimine (PEI) and collagen-based scaffolds. (2011)

Abstract The healing potential of scaffolds for tissue engineering can be enhanced by combining them with genes to produce gene-activated matrices (GAMs) for tissue regeneration. We examined the potential of using polyethyleneimine (PEI) as a vector for transfection of mesenchymal stem cells (MSCs) in monolayer culture and in 3D collagen-based GAMs. PEI-pDNA polyplexes were fabricated at a range of N/P ratios and their optimal transfection parameters (N/P 7 ratio, 2μg dose) and transfection efficiencies (30±8%) determined in monolayer culture. The polyplexes were then loaded onto collagen, collagen-glycosaminoglycan and collagen-nanohydroxyapatite scaffolds where gene expression was observed up to 21 days with a polyplex dose as low as 2μg. Transient expression profiles indicated that the GAMs act as a polyplex depot system whereby infiltrating cells become transfected over time as they migrate throughout the scaffold. The collagen-nHa GAM exhibited the most prolonged and elevated levels of transgene expression. This research has thus demonstrated that PEI is a highly efficient pDNA transfection agent for both MSC monolayer cultures and in the 3D GAM environment. By combining therapeutic gene therapy with highly engineered scaffolds, it is proposed that these GAMs might have immense capability to promote tissue regeneration.
Collections Ireland -> Royal College of Surgeons in Ireland -> PubMed

Full list of authors on original publication

Fergal J O'Brien, Sally-Ann Cryan, Alan J Hibbitts, Garry P Duffy, Erica G Tierney

Experts in our system

1
Fergal J O'Brien
Royal College of Surgeons in Ireland
Total Publications: 265
 
2
Sally-Ann Cryan
Royal College of Surgeons in Ireland
Total Publications: 67
 
3
Alan Hibbitts
Royal College of Surgeons in Ireland
Total Publications: 9
 
4
Garry P Duffy
Royal College of Surgeons in Ireland
Total Publications: 54
 
5
Erica G Tierney
Royal College of Surgeons in Ireland
Total Publications: 9