Journal Article


L M McNamara
F J O'Brien
O Brennan



staining and labeling antagonists inhibitors animals estrogen antagonists receptors estrogen mice metabolism osteocalcin osteopontin indoles anthraquinones estrogens estradiol minerals cell line dapi cell count osteoblasts fulvestrant alizarin humans pharmacology drug effects analogs derivatives

Estrogen plus estrogen receptor antagonists alter mineral production by osteoblasts in vitro. (2011)

Abstract In early postmenopausal women, estrogen withdrawal is associated with increased bone turnover leading to bone loss and increased risk of fracture. Recent studies have suggested that the remaining bone tissue is significantly stronger, stiffer and has an increased tissue-level mineral content. Such changes may occur to compensate for bone loss or as a direct result of estrogen deficiency. To date many details of the physiology of osteoblastic cells during estrogen deficiency are vague. In this study we test the hypothesis that osteoblastic matrix mineralisation is altered at the onset of estrogen deficiency. In vitro cell culture experiments were carried out up to 28 days to compare the mineral production of MC3T3-E1 osteoblastic cells subject to estrogen deficiency (fulvestrant), enhanced estrogen supplementation (17-β-estradiol) or a combination of both. Mineralisation was detected using von Kossa staining and was quantified with alizarin red absorbance readings. The expression of osteocalcin and osteopontin proteins, markers of osteoblast differentiation and mineralisation, was monitored using immunohistochemistry. Our results demonstrate that estrogen enhancement improves matrix mineralisation by MC3T3 cells in vitro. Furthermore this study found a significant reduction in the level of mineralisation when cells were treated with a combination of estrogen and fulvestrant. In an estrogen deficient environment mineralisation by osteoblastic cells was not altered. These findings suggest that altered tissue mineralisation following estrogen deficiency is not a direct result of estrogen deficiency on osteoblasts. Rather, we propose that altered tissue mineralisation may be a compensatory mechanism by bone to counter bone loss and reduced strength.
Collections Ireland -> Royal College of Surgeons in Ireland -> PubMed

Full list of authors on original publication

L M McNamara, F J O'Brien, O Brennan

Experts in our system

Laoise M McNamara
Royal College of Surgeons in Ireland
Total Publications: 7
Fergal J O'Brien
Royal College of Surgeons in Ireland
Total Publications: 265
Orlaith Brennan
Royal College of Surgeons in Ireland
Total Publications: 31