Type

Journal Article

Authors

Noel G McElvaney
Cedric Gunaratnam
Martin Clynes
Emmet O'Brien
Cormac McCarthy
Teck Boon Low
Michelle A Murray
Isabel Vega-Carrascal
Paula Meleady
Michael Henry
and 5 others

Subjects

Biochemistry

Topics
adult humans metabolism antigens cd cystic fibrosis female biological markers enzyme linked immunosorbent assay drug therapy alpha 1 antitrypsin proteomics disease progression fetal proteins alcam protein human male cell adhesion molecules neuronal young adult neutrophils

A novel neutrophil derived inflammatory biomarker of pulmonary exacerbation in cystic fibrosis. (2011)

Abstract The focus of this study was to characterize a novel biomarker for cystic fibrosis (CF) that could reflect exacerbations of the disease and could be useful for therapeutic stratification of patients, or for testing of potential drug treatments. This study focused exclusively on a protein complex containing alpha-1 antitrypsin and CD16b (AAT:CD16b) which is released into the bloodstream from membranes of pro-inflammatory primed neutrophils. Neutrophil membrane expression and extracellular levels of AAT and CD16b were quantified by flow cytometry, Western blot analysis and by 2D-PAGE. Interleukin-8 (IL-8), tumor necrosis factor-alpha (TNF-alpha) and AAT:CD16b complex were quantified in CF plasma (n=38), samples post antibiotic treatment for 14 days (n=10), chronic obstructive pulmonary disease (n=10), AAT deficient (n=10) and healthy control (n=14) plasma samples by ELISA. Cell priming with IL-8 and TNF-alpha caused release of the AAT:CD16b complex from the neutrophil cell membrane. Circulating plasma levels of IL-8, TNF-alpha and AAT:CD16b complex were significantly higher in patients with CF than in the other patient groups or healthy controls (P<0.05). Antibiotic treatment of pulmonary exacerbation in patients with CF led to decreased plasma protein concentrations of AAT:CD16b complex with a significant correlation with improved FEV1 (r=0.81, P=0.003). The results of this study have shown that levels of AAT:CD16b complex present in plasma correlate to the inflammatory status of patients. The AAT:CD16b biomarker may become a useful addition to the clinical diagnosis of exacerbations in CF.
Collections Ireland -> Royal College of Surgeons in Ireland -> PubMed

Full list of authors on original publication

Noel G McElvaney, Cedric Gunaratnam, Martin Clynes, Emmet O'Brien, Cormac McCarthy, Teck Boon Low, Michelle A Murray, Isabel Vega-Carrascal, Paula Meleady, Michael Henry and 5 others

Experts in our system

1
Noel G McElvaney
Royal College of Surgeons in Ireland
Total Publications: 190
 
2
Cedric Gunaratnam
Royal College of Surgeons in Ireland
Total Publications: 18
 
3
Martin Clynes
Dublin City University
Total Publications: 209
 
4
Cormac McCarthy
Royal College of Surgeons in Ireland
Total Publications: 19
 
5
Michelle A Murray
Royal College of Surgeons in Ireland
Total Publications: 8
 
6
Paula Meleady
Dublin City University
Total Publications: 95
 
7
Michael Henry
Dublin City University
Total Publications: 78