Journal Article


David C Henshall
Raymond L Stallings
Roger P Simon
Julie A Saugstad
Takanori Sano
Katsuhiro Tanaka
Genshin Mouri
Ross C McKiernan
Tobias Engel
Amaya Sanz-Rodriguez
and 2 others



kainic acid mice inbred c57bl eif2c2 protein mouse antagomir 122 argonaute proteins mirn132 microrna mouse up regulation excitatory amino acid agonists antagonists inhibitors male oligonucleotides mice status epilepticus metabolism amygdala pharmacology micrornas toxicity animals injections intralesional hippocampus prevention control down regulation

miRNA Expression profile after status epilepticus and hippocampal neuroprotection by targeting miR-132. (2011)

Abstract When an otherwise harmful insult to the brain is preceded by a brief, noninjurious stimulus, the brain becomes tolerant, and the resulting damage is reduced. Epileptic tolerance develops when brief seizures precede an episode of prolonged seizures (status epilepticus). MicroRNAs (miRNAs) are small, noncoding RNAs that function as post-transcriptional regulators of gene expression. We investigated how prior seizure preconditioning affects the miRNA response to status epilepticus evoked by intra-amygdalar kainic acid in mice. The miRNA was extracted from the ipsilateral CA3 subfield 24 hours after focal-onset status epilepticus in animals that had previously received either seizure preconditioning (tolerance) or no preconditioning (injury), and mature miRNA levels were measured using TaqMan low-density arrays. Expression of 21 miRNAs was increased, relative to control, after status epilepticus alone, and expression of 12 miRNAs was decreased. Increased miR-132 levels were matched with increased binding to Argonaute-2, a constituent of the RNA-induced silencing complex. In tolerant animals, expression responses of >40% of the injury-group-detected miRNAs differed, being either unchanged relative to control or down-regulated, and this included miR-132. In vivo microinjection of locked nucleic acid-modified oligonucleotides (antagomirs) against miR-132 depleted hippocampal miR-132 levels and reduced seizure-induced neuronal death. Thus, our data strongly suggest that miRNAs are important regulators of seizure-induced neuronal death.
Collections Ireland -> Royal College of Surgeons in Ireland -> PubMed

Full list of authors on original publication

David C Henshall, Raymond L Stallings, Roger P Simon, Julie A Saugstad, Takanori Sano, Katsuhiro Tanaka, Genshin Mouri, Ross C McKiernan, Tobias Engel, Amaya Sanz-Rodriguez and 2 others

Experts in our system

David C Henshall
Royal College of Surgeons in Ireland
Total Publications: 127
Raymond L Stallings
Royal College of Surgeons in Ireland
Total Publications: 59
Tobias Engel
Royal College of Surgeons in Ireland
Total Publications: 66
Amaya Sanz-Rodriguez
Royal College of Surgeons in Ireland
Total Publications: 18