Type

Journal Article

Authors

Ann M Hopkins
Arnold D K Hill
Maurice Stokes
Malcolm R Kell
Peter A Dervan
Martin J Shelly
Igor Aurrekoetxea
Alfonso Blanco
David C Cottell
Lance Hudson
and 1 others

Subjects

Biochemistry

Topics
tumor markers biological vimentin cell proliferation cell culture techniques pathology breast neoplasms ultrastructure tumor cells cultured humans cell shape actins keratins type i flotillins cell aging female membrane proteins neoplastic stem cells metabolism

An imbalance in progenitor cell populations reflects tumour progression in breast cancer primary culture models. (2011)

Abstract Many factors influence breast cancer progression, including the ability of progenitor cells to sustain or increase net tumour cell numbers. Our aim was to define whether alterations in putative progenitor populations could predict clinicopathological factors of prognostic importance for cancer progression. Primary cultures were established from human breast tumour and adjacent non-tumour tissue. Putative progenitor cell populations were isolated based on co-expression or concomitant absence of the epithelial and myoepithelial markers EPCAM and CALLA respectively. Significant reductions in cellular senescence were observed in tumour versus non-tumour cultures, accompanied by a stepwise increase in proliferation:senescence ratios. A novel correlation between tumour aggressiveness and an imbalance of putative progenitor subpopulations was also observed. Specifically, an increased double-negative (DN) to double-positive (DP) ratio distinguished aggressive tumours of high grade, estrogen receptor-negativity or HER2-positivity. The DN:DP ratio was also higher in malignant MDA-MB-231 cells relative to non-tumorigenic MCF-10A cells. Ultrastructural analysis of the DN subpopulation in an invasive tumour culture revealed enrichment in lipofuscin bodies, markers of ageing or senescent cells. Our results suggest that an imbalance in tumour progenitor subpopulations imbalances the functional relationship between proliferation and senescence, creating a microenvironment favouring tumour progression.
Collections Ireland -> Royal College of Surgeons in Ireland -> PubMed

Full list of authors on original publication

Ann M Hopkins, Arnold D K Hill, Maurice Stokes, Malcolm R Kell, Peter A Dervan, Martin J Shelly, Igor Aurrekoetxea, Alfonso Blanco, David C Cottell, Lance Hudson and 1 others

Experts in our system

1
Ann M Hopkins
Royal College of Surgeons in Ireland
Total Publications: 26
 
2
Arnold D K Hill
Royal College of Surgeons in Ireland
Total Publications: 110
 
3
Lance Hudson
Royal College of Surgeons in Ireland
Total Publications: 11