Type

Journal Article

Authors

Noel G McElvaney
Peter Greally
Shane J O'Neill
Michael Williamson
Emer P Reeves

Subjects

Microbiology

Topics
glycosaminoglycans interleukin 8 young adult drug effects blotting western humans inflammation middle aged adult sputum cystic fibrosis nebulizers and vaporizers saline solution hypertonic administration inhalation immunology complications administration dosage enzyme linked immunosorbent assay bronchoalveolar lavage fluid drug therapy electrophoresis polyacrylamide gel

Nebulized hypertonic saline decreases IL-8 in sputum of patients with cystic fibrosis. (2011)

Abstract Inflammation within the cystic fibrosis (CF) lung is mediated by inflammatory chemokines, such as IL-8. IL-8 is protected from proteolytic degradation in the airways by binding to glycosaminoglycans, while remaining active. Evidence that increased hypertonicity of airway secretions induced by hypertonic saline treatment alters levels of IL-8 is lacking. To investigate the antiinflammatory effect of hypertonic saline (HTS) treatment within the CF lung by focusing on IL-8. Degradation of IL-8 in CF lung secretions after treatment with glycosaminoglycan lyases and HTS was analyzed by Western blot analysis and ELISA. The ex vivo chemotactic activity of purified neutrophils in response to CF airway secretions was evaluated post nebulization of HTS (7% saline). In vivo CF bronchoalveolar lavage fluid (BALF) IL-8 levels were significantly higher than the control group (P < 0.05). Digesting glycosaminoglycans in CF BALF displaced IL-8 from glycosaminoglycan matrices, rendering the chemokine susceptible to proteolytic cleavage. High sodium concentrations also liberate IL-8 in CF BALF in vitro, and in vivo in CF sputum from patients receiving aerosolized HTS, resulting in degradation of IL-8 and decreased neutrophil chemotactic efficiency. Glycosaminoglycans possess the ability to influence the chemokine profile of the CF lung by binding and stabilizing IL-8, which promotes neutrophil chemotaxis and activation. Nebulized hypertonic saline treatment disrupts the interaction between glycosaminoglycans and IL-8, rendering IL-8 susceptible to proteolytic degradation with subsequent decrease in neutrophil chemotaxis, thereby facilitating resolution of inflammation.
Collections Ireland -> Royal College of Surgeons in Ireland -> PubMed

Full list of authors on original publication

Noel G McElvaney, Peter Greally, Shane J O'Neill, Michael Williamson, Emer P Reeves

Experts in our system

1
Noel G McElvaney
Royal College of Surgeons in Ireland
Total Publications: 194
 
2
Peter Greally
TU Dublin (Tallaght Campus)
Total Publications: 30
 
3
Shane J O'Neill
Royal College of Surgeons in Ireland
Total Publications: 84
 
4
Emer P Reeves
Royal College of Surgeons in Ireland
Total Publications: 63