Journal Article


Fergal J O'Brien
Ryan J McCoy



bone and bones instrumentation bioreactors tissue scaffolds physiology perfusion chemistry cell culture techniques stress mechanical

Influence of shear stress in perfusion bioreactor cultures for the development of three-dimensional bone tissue constructs: a review. (2010)

Abstract Bone tissue engineering aims to generate clinically applicable bone graft substitutes in an effort to ease the demands and reduce the potential risks associated with traditional autograft and allograft bone replacement procedures. Biomechanical stimuli play an important role under physiologically relevant conditions in the normal formation, development, and homeostasis of bone tissue--predominantly, strain (predicted levels in vivo for humans <2000 με) caused by physical deformation, and fluid shear stress (0.8-3 Pa), generated by interstitial fluid movement through lacunae caused by compression and tension under loading. Therefore, in vitro bone tissue cultivation strategies seek to incorporate biochemical stimuli in an effort to create more physiologically relevant constructs for grafting. This review is focused on collating information pertaining to the relationship between fluid shear stress, cellular deformation, and osteogenic differentiation, providing further insight into the optimal culture conditions for the creation of bone tissue substitutes.
Collections Ireland -> Royal College of Surgeons in Ireland -> PubMed

Full list of authors on original publication

Fergal J O'Brien, Ryan J McCoy

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Fergal J O'Brien
Royal College of Surgeons in Ireland
Total Publications: 265
Ryan J McCoy
Royal College of Surgeons in Ireland