Type

Journal Article

Authors

Noel G McElvaney
Clifford C Taggart
Shane J O'Neill
Rodney L Levine
Efrat Kessler
Sinéad Weldon
Catherine M Greene
Marcus W Butler
Gudmundur Bergsson
Nicolas Guyot

Subjects

Biochemistry

Topics
pseudomonas aeruginosa blotting western bacterial proteins metabolism fibronectins humans chromatography high pressure liquid mass spectrometry enzymology electrophoresis polyacrylamide gel elafin metalloproteases

Functional study of elafin cleaved by Pseudomonas aeruginosa metalloproteinases. (2010)

Abstract Elafin is a 6-kDa innate immune protein present at several epithelial surfaces including the pulmonary epithelium. It is a canonical protease inhibitor of two neutrophil serine proteases [neutrophil elastase (NE) and proteinase 3] with the capacity to covalently bind extracellular matrix proteins by transglutamination. In addition to these properties, elafin also possesses antimicrobial and immunomodulatory activities. The aim of the present study was to investigate the effect of Pseudomonas aeruginosa proteases on elafin function. We found that P. aeruginosa PAO1-conditioned medium and two purified Pseudomonas metalloproteases, pseudolysin (elastase) and aeruginolysin (alkaline protease), are able to cleave recombinant elafin. Pseudolysin was shown to inactivate the anti-NE activity of elafin by cleaving its protease-binding loop. Interestingly, antibacterial properties of elafin against PAO1 were found to be unaffected after pseudolysin treatment. In contrast to pseudolysin, aeruginolysin failed to inactivate the inhibitory properties of elafin against NE. Aeruginolysin cleaves elafin at the amino-terminal Lys6-Gly7 peptide bond, resulting in a decreased ability to covalently bind purified fibronectin following transglutaminase activity. In conclusion, this study provides evidence that elafin is susceptible to proteolytic cleavage at alternative sites by P. aeruginosa metalloproteinases, which can affect different biological functions of elafin.
Collections Ireland -> Royal College of Surgeons in Ireland -> PubMed

Full list of authors on original publication

Noel G McElvaney, Clifford C Taggart, Shane J O'Neill, Rodney L Levine, Efrat Kessler, Sinéad Weldon, Catherine M Greene, Marcus W Butler, Gudmundur Bergsson, Nicolas Guyot

Experts in our system

1
Noel G McElvaney
Royal College of Surgeons in Ireland
Total Publications: 194
 
2
Clifford C Taggart
Royal College of Surgeons in Ireland
Total Publications: 54
 
3
Shane J O'Neill
Royal College of Surgeons in Ireland
Total Publications: 84
 
4
Sinéad Weldon
Royal College of Surgeons in Ireland
Total Publications: 9
 
5
Catherine M Greene
Royal College of Surgeons in Ireland
Total Publications: 150
 
6
Marcus W Butler
Royal College of Surgeons in Ireland
Total Publications: 9