Journal Article


Jacqueline S Daly
Fergal J O'Brien
Michael B Keogh



cell proliferation glycosaminoglycans cytology drug effects cells cultured therapeutic use biological markers staining and labeling therapy humans tissue culture techniques cell movement collagen metabolism phenotype osteoblasts bone regeneration tissue engineering calcification physiologic dose response relationship drug bone diseases physiology pharmacology osteogenesis time factors biocompatible materials transforming growth factor beta1 cell adhesion tissue scaffolds

A novel collagen scaffold supports human osteogenesis--applications for bone tissue engineering. (2009)

Abstract Collagen glycosaminoglycan (CG) scaffolds have been clinically approved as an application for skin regeneration. The goal of this study has been to examine whether a CG scaffold is a suitable biomaterial for generating human bone tissue. Specifically, we have asked the following questions: (1) can the scaffold support human osteoblast growth and differentiation and (2) how might recombinant human transforming growth factor-beta (TGF-beta(1)) enhance long-term in vitro bone formation? We show human osteoblast attachment, infiltration and uniform distribution throughout the construct, reaching the centre within 14 days of seeding. We have identified the fully differentiated osteoblast phenotype categorised by the temporal expression of alkaline phosphatase, collagen type 1, osteonectin, bone sialo protein, biglycan and osteocalcin. Mineralised bone formation has been identified at 35 days post-seeding by using von Kossa and Alizarin S Red staining. Both gene expression and mineral staining suggest the benefit of introducing an initial high treatment of TGF-beta(1) (10 ng/ml) followed by a low continuous treatment (0.2 ng/ml) to enhance human osteogenesis on the scaffold. Osteogenesis coincides with a reduction in scaffold size and shape (up to 70% that of original). A notable finding is core degradation at the centre of the tissue-engineered construct after 49 days of culture. This is not observed at earlier time points. Therefore, a maximum of 35 days in culture is appropriate for in vitro studies of these scaffolds. We conclude that the CG scaffold shows excellent potential as a biomaterial for human bone tissue engineering.
Collections Ireland -> Royal College of Surgeons in Ireland -> PubMed

Full list of authors on original publication

Jacqueline S Daly, Fergal J O'Brien, Michael B Keogh

Experts in our system

Jacqueline Daly
Royal College of Surgeons in Ireland
Total Publications: 12
Fergal J O'Brien
Royal College of Surgeons in Ireland
Total Publications: 265
Michael B Keogh
Royal College of Surgeons in Ireland
Total Publications: 7