Type

Journal Article

Authors

Heinrich Huber
Jochen Prehn
Peter Wellstead
Markus Rehm
Dimitrios Kalamatianos
Perrine Paul
Heiko Düssmann
Jakub Wenus

Subjects

Biochemistry

Topics
mitochondrial membranes time factors process control computer graphics software signal transduction fluorescence resonance energy transfer imaging three dimensional apoptosis live cell imaging online systems metabolism automation cytology cell survival software process cell line cells user computer interface design framework image analysis time series mitochondria hamilton institute

ALISSA: an automated live-cell imaging system for signal transduction analyses. (2009)

Abstract Probe photobleaching and a specimen's sensitivity to phototoxicity severely limit the number of possible excitation cycles in time-lapse fluorescent microscopy experiments. Consequently, when a study of cellular processes requires measurements over hours or days, temporal resolution is limited, and spontaneous or rapid events may be missed, thus limiting conclusions about transduction events. We have developed ALISSA, a design framework and reference implementation for an automated live-cell imaging system for signal transduction analysis. It allows an adaptation of image modalities and laser resources tailored to the biological process, and thereby extends temporal resolution from minutes to seconds. The system employs online image analysis to detect cellular events that are then used to exercise microscope control. It consists of a reusable image analysis software for cell segmentation, tracking, and time series extraction, and a measurement-specific process control software that can be easily adapted to various biological settings. We have applied the ALISSA framework to the analysis of apoptosis as a demonstration case for slow onset and rapid execution signaling. The demonstration provides a clear proof-of-concept for ALISSA, and offers guidelines for its application in a broad spectrum of signal transduction studies.
Collections Ireland -> Royal College of Surgeons in Ireland -> PubMed
Ireland -> Maynooth University -> Type = Article
Ireland -> Maynooth University -> Academic Unit = Faculty of Science and Engineering: Research Institutes: Hamilton Institute
Ireland -> Maynooth University -> Status = Published

Full list of authors on original publication

Heinrich Huber, Jochen Prehn, Peter Wellstead, Markus Rehm, Dimitrios Kalamatianos, Perrine Paul, Heiko Düssmann, Jakub Wenus

Experts in our system

1
Heinrich J Huber
Royal College of Surgeons in Ireland
Total Publications: 39
 
2
Jochen H M Prehn
Royal College of Surgeons in Ireland
Total Publications: 206
 
3
Markus Rehm
Royal College of Surgeons in Ireland
Total Publications: 55
 
4
Heiko Düssmann
Royal College of Surgeons in Ireland
Total Publications: 45