Journal Article


David J Bouchier-Hayes
Arnold D K Hill
Cathal Kelly
Claire Condron
Hong Chen
Tom G Neilan
Gang Chen



pharmacology metabolism quercetin ventricular dysfunction left antioxidants hsp72 heat shock proteins toxicity blood pressure lipopolysaccharides ischemic preconditioning myocardial animals chemically induced mice disease models animal heart rate mice inbred c57bl drug effects drug therapy glutamine

Attenuation of lipopolysaccharide-mediated left ventricular dysfunction by glutamine preconditioning. (2008)

Abstract Myocardial dysfunction is often seen during the inflammatory response to major surgery at 4 to 6h postoperatively. The aim of this study was to investigate the effect of glutamine pretreatment, as a means of preconditioning, on lipopolysaccharide-induced myocardial dysfunction. C57BL/6 mice were randomized into four groups: Control; lipopolysaccharide; glutamine plus lipopolysaccharide; and Quercetin, an inhibitor of heat shock protein synthesis plus glutamine and lipopolysaccharide. Left ventricular function was assessed at 6h following lipopolysaccharide (LPS) insult by invasive hemodynamics. Heat shock protein (HSP)72 in heart tissue was determined by Western immunoblot at 12h after glutamine administration. Administration of lipopolysaccharide resulted in significant decrease in left ventricular end systolic pressure (LVESP) (69.1 +/- 2.52 mm Hg versus 106.3 +/- 3.36 mm Hg in controls), reduced dP/dtmax (4704.1 +/- 425.31 mm Hg/s versus 9389.8 +/- 999.4 mm Hg/s in controls), and the increase in left ventricular end diastolic pressure (LVEDP) (5.10 +/- 0.28 mm Hg versus 2.16 +/- 0.27 mm Hg in controls) (P < 0.05). Peritoneal injection of 25 g/kg of glutamine 12 h prior to lipopolysaccharide exposure induced HSP72 expression in heart tissues and attenuated lipopolysaccharide-induced left ventricular dysfunction: LVESP 85.94 +/- 3.8 mm Hg (P < 0.05), dP/dtmax 8331 +/- 425 mm Hg (P < 0.05), LVEDP 2.32 +/- 0.23 mm Hg (P < 0.01). Quercetin partially attenuated glutamine induced HSP72 expression and blocked the protective response of glutamine. These data demonstrate that cardioprotection with glutamine is associated with induction of HSP72 and may be an approach to activating the preconditioning response in the heart in clinical practise.
Collections Ireland -> Royal College of Surgeons in Ireland -> PubMed

Full list of authors on original publication

David J Bouchier-Hayes, Arnold D K Hill, Cathal Kelly, Claire Condron, Hong Chen, Tom G Neilan, Gang Chen

Experts in our system

Arnold D K Hill
Royal College of Surgeons in Ireland
Total Publications: 110
G Chen
Royal College of Surgeons in Ireland
Total Publications: 19