Journal Article


Fergal J O'Brien
Joachim Hammer
Tanya Levingstone
Christian Jungreuthmayer
Tim Weber
John P Gleeson
Niamh A Plunkett
Amir A Al-Munajjed



animals tissue scaffolds 3t3 cells chemistry durapatite bone and bones spectroscopy fourier transform infrared collagen biomimetics tissue engineering porosity mice pathology bone substitutes body fluids

Development of a biomimetic collagen-hydroxyapatite scaffold for bone tissue engineering using a SBF immersion technique. (2009)

Abstract The objective of this study was to develop a biomimetic, highly porous collagen-hydroxyapatite (HA) composite scaffold for bone tissue engineering (TE), combining the biological performance and the high porosity of a collagen scaffold with the high mechanical stiffness of a HA scaffold. Pure collagen scaffolds were produced using a lyophilization process and immersed in simulated body fluid (SBF) to provide a biomimetic coating. Pure collagen scaffolds served as a control. The mechanical, material, and structural properties of the scaffolds were analyzed and the biological performance of the scaffolds was evaluated by monitoring the cellular metabolic activity and cell number at 1, 2, and 7 days post seeding. The SBF-treated scaffolds exhibited a significantly increased stiffness compared to the pure collagen group (4-fold increase), while a highly interconnected structure (95%) was retained. FTIR indicated that the SBF coating exhibited similar characteristics to pure HA. Micro-CT showed a homogeneous distribution of HA. Scanning electron microscopy also indicated a mineralization of the collagen combined with a precipitation of HA onto the collagen. The excellent biological performance of the collagen scaffolds was maintained in the collagen-HA scaffolds as demonstrated from cellular metabolic activity and total cell number. This investigation has successfully developed a biomimetic collagen-HA composite scaffold. An increase in the mechanical properties combined with an excellent biological performance in vitro was observed, indicating the high potential of the scaffold for bone TE.
Collections Ireland -> Royal College of Surgeons in Ireland -> PubMed

Full list of authors on original publication

Fergal J O'Brien, Joachim Hammer, Tanya Levingstone, Christian Jungreuthmayer, Tim Weber, John P Gleeson, Niamh A Plunkett, Amir A Al-Munajjed

Experts in our system

Fergal J O'Brien
Royal College of Surgeons in Ireland
Total Publications: 265
Tanya J Levingstone
Royal College of Surgeons in Ireland
Total Publications: 28
John P Gleeson
Royal College of Surgeons in Ireland
Total Publications: 26