Type

Journal Article

Authors

Jochen H M Prehn
David C Henshall
Nikolaus Plesnila
Waro Taki
Ina Woods
Tobias Engel
Caoimhín G Concannon
Isabelle Virard
Katsura Kuroki

Subjects

Biochemistry

Topics
cerebral cortex rna messenger time factors puma protein mouse animals corpus striatum mice drug effects mice knockout tumor suppressor proteins infarction middle cerebral artery blood supply cerebral infarction genetics metabolism signal transduction up regulation disease models animal pathology disability evaluation brain ischemia nerve degeneration mice inbred c57bl apoptosis regulatory proteins cell death physiology physiopathology

Effects of transient focal cerebral ischemia in mice deficient in puma. (2008)

Abstract Bcl-2 homology domain 3 (BH3)-only pro-apoptotic proteins may play an important role in upstream cell death signaling pathways underlying ischemic brain injury. Puma is a potent BH3-only protein that can be induced via p53, FoxO3a and endoplasmic reticulum stress pathways and is upregulated by global cerebral ischemia. To more completely define the contribution of Puma to ischemic brain injury we measured the expressional response of Puma to transient focal cerebral ischemia in mice and also compared infarct volumes in puma-deficient versus puma-expressing mice. Real-time quantitative PCR determined puma mRNA levels were significantly increased 8h after 90min middle cerebral artery (MCA) occlusion in the ipsilateral cortex, while expression remained unchanged contralaterally. Puma protein levels were also increased in the ischemic cortex over the same period. However, cortical and striatal infarct volumes were not significantly different between puma-deficient and puma-expressing mice at 24h, and no differences between genotypes were found for post-ischemic neurological deficit scores. These data demonstrate that focal cerebral ischemia is associated with puma induction but suggest that Puma does not contribute significantly to lesion development in the present model.
Collections Ireland -> Royal College of Surgeons in Ireland -> PubMed

Full list of authors on original publication

Jochen H M Prehn, David C Henshall, Nikolaus Plesnila, Waro Taki, Ina Woods, Tobias Engel, Caoimhín G Concannon, Isabelle Virard, Katsura Kuroki

Experts in our system

1
Jochen H M Prehn
Royal College of Surgeons in Ireland
Total Publications: 206
 
2
David C Henshall
Royal College of Surgeons in Ireland
Total Publications: 127
 
3
Nikolaus Plesnila
Royal College of Surgeons in Ireland
Total Publications: 9
 
4
Ina Woods
Royal College of Surgeons in Ireland
Total Publications: 16
 
5
Tobias Engel
Royal College of Surgeons in Ireland
Total Publications: 66
 
6
Caoimhín G Concannon
Royal College of Surgeons in Ireland
Total Publications: 46