Type

Journal Article

Authors

D R Cotter
G Cagney
M J Dunn
C Byrne
A T Behan

Subjects

Biochemistry

Topics
chromatography liquid standards adult humans nerve tissue proteins repressor proteins female receptors transferrin proteomics metabolism flotillins male limbic system associated membrane protein basp1 protein human aged middle aged bipolar disorder membrane proteins electrophoresis gel two dimensional tandem mass spectrometry blotting western schizophrenia reproducibility of results matrix assisted laser electrophoresis polyacrylamide gel cell adhesion molecules neuronal gpi linked proteins prefrontal cortex mass spectrometry stxbp1 protein human munc18 proteins

Proteomic analysis of membrane microdomain-associated proteins in the dorsolateral prefrontal cortex in schizophrenia and bipolar disorder reveals alterations in LAMP, STXBP1 and BASP1 protein expression. (2008)

Abstract The dorsolateral prefrontal cortex (dlpfc) is strongly implicated in the pathogenesis of schizophrenia (SCZ) and bipolar disorder (BPD) and, within this region, abnormalities in glutamatergic neurotransmission and synaptic function have been described. Proteins associated with these functions are enriched in membrane microdomains (MM). In the current study, we used two complementary proteomic methods, two-dimensional difference gel electrophoresis and one-dimensional sodium dodecyl sulphate polyacrylamide gel electrophoresis followed by reverse phase-liquid chromatography-tandem mass spectrometry (RP-LC-MS/MS) (gel separation liquid chromatography-tandem mass spectrometry (GeLC-MS/MS)) to assess protein expression in MM in pooled samples of dlpfc from SCZ, BPD and control cases (n=10 per group) from the Stanley Foundation Brain series. We identified 16 proteins altered in one/both disorders using proteomic methods. We selected three proteins with roles in synaptic function (syntaxin-binding protein 1 (STXBP1), brain abundant membrane-attached signal protein 1 (BASP1) and limbic system-associated membrane protein (LAMP)) for validation by western blotting. This revealed significantly increased expression of these proteins in SCZ (STXBP1 (24% difference; P<0.001), BASP1 (40% difference; P<0.05) and LAMP (22% difference; P<0.01)) and BPD (STXBP1 (31% difference; P<0.001), BASP1 (23% difference; P<0.01) and LAMP (20% difference; P<0.01)) in the Stanley brain series (n=20 per group). Further validation in dlpfc from the Harvard brain subseries (n=10 per group) confirmed increased protein expression in SCZ of STXBP1 (18% difference; P<0.0001), BASP1 (14% difference; P<0.0001) but not LAMP (20% difference; P=0.14). No significant differences in STXBP1, BASP1 or LAMP protein expression in BPD dlpfc were observed. This study, through proteomic assessments of MM in dlpfc and validation in two brain series, strongly implicates LAMP, STXBP1 and BASP1 in SCZ and supports the view of a neuritic and synaptic dysfunction in the neuropathology of SCZ.
Collections Ireland -> Royal College of Surgeons in Ireland -> PubMed

Full list of authors on original publication

D R Cotter, G Cagney, M J Dunn, C Byrne, A T Behan

Experts in our system

1
David R Cotter
Royal College of Surgeons in Ireland
Total Publications: 56
 
2
Gerard Cagney
Royal College of Surgeons in Ireland
Total Publications: 54
 
3
Christopher Byrne
Royal College of Surgeons in Ireland
Total Publications: 13