Type

Journal Article

Authors

Norman Delanty
David Goldstein
Peter Kinirons
Mary McCarthy
Pat Dicker
Clare Muckian
Gianpiero L Cavalleri
Colin Doherty
Kevin Murphy
Amre Shahwan

Subjects

Microbiology

Topics
polymorphism single nucleotide abcb1 protein human therapeutic use linkage disequilibrium adult anticonvulsants ireland humans drug resistance p glycoprotein introns gene frequency epilepsy exons genotype genetics organic anion transporters drug therapy

The controversial association of ABCB1 polymorphisms in refractory epilepsy: an analysis of multiple SNPs in an Irish population. (2006)

Abstract Controversy has surrounded the reported association of the single nucleotide polymorphism (SNP) C3435T of the ATP-binding cassette subfamily B member 1 (ABCB1, MDR1) gene, with refractory epilepsy. Here we examine this question by: (1) attempting to replicate the original association, (2) assessing the association of other variants in high linkage disequilibrium (LD) with C3435T, and (3) evaluating and comparing our findings with other published studies. We defined drug-responsiveness as seizure freedom or a 50% or more reduction in seizure frequency in the preceding year. Drug resistance was defined as a less than 50% reduction in seizure frequency in the preceding year. We used a combination of map-based (tagging SNPs) and direct sequence-based methods allowing a comprehensive assessment of variation across the associated interval. Genotypic data on 8 SNPs was collected on 440 patients, of whom 242 were drug-responsive and 198 were drug-resistant. We were unable to observe the original association of drug-resistant epilepsy with C3435T, nor any association with other functional variants at SNP or haplotype level in the ABCB1 gene. Evaluation of other attempted replication studies suggest that if the original C3435T association is indeed real, it would appear highly sensitive to the phenotype used. Alternatively, the discrepant results of this and other association attempts may be indicative of the original report of the CC genotype at C3435T in ABCB1 being a false positive finding. Variability in phenotypic descriptions in genetic association studies may partly explain the inconsistency of attempted replications.
Collections Ireland -> Royal College of Surgeons in Ireland -> PubMed

Full list of authors on original publication

Norman Delanty, David Goldstein, Peter Kinirons, Mary McCarthy, Pat Dicker, Clare Muckian, Gianpiero L Cavalleri, Colin Doherty, Kevin Murphy, Amre Shahwan

Experts in our system

1
Norman Delanty
Royal College of Surgeons in Ireland
Total Publications: 41
 
2
David B Goldstein
Royal College of Surgeons in Ireland
 
3
Gianpiero L Cavalleri
Royal College of Surgeons in Ireland
Total Publications: 35
 
4
Colin P Doherty
Trinity College Dublin
Total Publications: 35