Type

Journal Article

Authors

Noel G McElvaney
Clifford C Taggart
Shane J O'Neill
Catherine M Greene
Tomás P Carroll
Siobhan Griffin

Subjects

Biochemistry

Topics
drug effects genetics enzyme linked immunosorbent assay models biological cell line tumor receptor epidermal growth factor mucins secretory leukocyte peptidase inhibitor humans metabolism gene expression pharmacology lipopolysaccharides reverse transcriptase polymerase chain reaction leukocyte elastase

Effect of pro-inflammatory stimuli on mucin expression and inhibition by secretory leucoprotease inhibitor. (2006)

Abstract Stimuli-induced expression of certain mucin genes has been demonstrated to occur as a result of ligand-dependent activation of the epidermal growth factor receptor (EGFR). In particular, MUC5AC expression can be induced by cigarette-smoke, neutrophil elastase and lipopolysaccharide (LPS) following activation of tumour necrosis factor alpha-converting enzyme. We now show that a large of number of stimuli relevant to the cystic fibrosis lung - neutrophil elastase, LPS, Pam3Cys-Ser-(Lys)4 Hydrochloride (a lipopeptide analogue), CpG DNA (which mimics bacterial DNA) and cystic fibrosis bronchoalveolar lavage fluid - can activate MUC1 and 2 expression as well as MUC5AC expression in lung epithelial cells via an EGFR-dependent mechanism. In addition, we demonstrate that the immunomodulatory anti-protease, secretory leucoprotease inhibitor, can inhibit stimuli-induced MUC1, 2 and 5AC expression via a mechanism that is primarily dependent on the inhibition of transforming growth factor type alpha release. Therefore, mucin gene expression, induced by cystic fibrosis respiratory stimuli, can be inhibited by secretory leucoprotease inhibitor indicating its potential importance as an anti-mucin agent in cystic fibrosis and other chronic lung diseases characterized by mucus hypersecretion.
Collections Ireland -> Royal College of Surgeons in Ireland -> PubMed

Full list of authors on original publication

Noel G McElvaney, Clifford C Taggart, Shane J O'Neill, Catherine M Greene, Tomás P Carroll, Siobhan Griffin

Experts in our system

1
Noel G McElvaney
Royal College of Surgeons in Ireland
Total Publications: 194
 
2
Clifford C Taggart
Royal College of Surgeons in Ireland
Total Publications: 54
 
3
Shane J O'Neill
Royal College of Surgeons in Ireland
Total Publications: 84
 
4
Catherine M Greene
Royal College of Surgeons in Ireland
Total Publications: 150
 
5
Tomás P Carroll
Royal College of Surgeons in Ireland
Total Publications: 26