Type

Journal Article

Authors

Noel G McElvaney
Clifford C Taggart
Shane J O'Neill
Catherine M Greene
Ian Robertson
Marcus W Butler

Subjects

Biochemistry

Topics
ubiquitin nf kappa b metabolism proteasome endopeptidase complex map kinase kinase 4 chemokine ccl2 lipopolysaccharides u937 cells elafin monocytes pharmacology drug effects humans ccl2 protein human transcription factor ap 1

Elafin prevents lipopolysaccharide-induced AP-1 and NF-kappaB activation via an effect on the ubiquitin-proteasome pathway. (2006)

Abstract The serine anti-protease elafin is expressed by monocytes, alveolar macrophages, neutrophils, and at mucosal surfaces and possesses antimicrobial activity. It is also known to reduce lipopolysaccharide-induced neutrophil influx into murine alveoli as well as to abrogate lipopolysaccharide-induced production of matrix metalloprotease 9, macrophage inhibitory protein 2, and tumor necrosis factor-alpha by as-yet unidentified mechanisms. In this report we have shown that elafin inhibits the lipopolysaccharide-induced production of monocyte chemoattractant protein-1 in monocytes by inhibiting AP-1 and NF-kappaB activation. Elafin prevented lipopolysaccharide-induced phosphorylation of AP-1, c-Jun, and JNK but had no effect on phosphorylation of p38. The lipopolysaccharide-induced degradation of IL-1R-associated kinase 1, IkappaBalpha, and IkappaBbeta was inhibited by elafin but phosphorylation of IkappaBalpha was unaffected. Polyubiquitinated protein including polyubiquitinated IkappaBalpha was shown to accumulate in the presence of elafin. These results suggest that inhibition by elafin of lipopolysaccharide-induced AP-1 and NF-kappaB activation occurs via an effect on the ubiquitin-proteasome pathway.
Collections Ireland -> Royal College of Surgeons in Ireland -> PubMed

Full list of authors on original publication

Noel G McElvaney, Clifford C Taggart, Shane J O'Neill, Catherine M Greene, Ian Robertson, Marcus W Butler

Experts in our system

1
Noel G McElvaney
Royal College of Surgeons in Ireland
Total Publications: 190
 
2
Clifford C Taggart
Royal College of Surgeons in Ireland
Total Publications: 54
 
3
Shane J O'Neill
Royal College of Surgeons in Ireland
Total Publications: 81
 
4
Catherine M Greene
Royal College of Surgeons in Ireland
Total Publications: 150
 
5
Ian H Robertson
Trinity College Dublin
Total Publications: 142
 
6
Marcus W Butler
Royal College of Surgeons in Ireland
Total Publications: 15