Type

Journal Article

Authors

Elaine W Kay
Mary B Leader
Anthony O'Grady
Fiona Lonergan
Robert Cummins

Subjects

Microbiology

Topics
diagnosis humans cyclin d1 tissue fixation antigens immunohistochemistry paraffin embedding formaldehyde lymphoma mantle cell genetics chemistry pathology

A simple antigen retrieval method for the optimal demonstration of cyclin-D1 overexpression in formalin-fixed paraffin-embedded cases of mantle cell lymphoma. (2005)

Abstract Successful diagnosis of mantle cell lymphoma (MCL) by immunohistochemistry is largely dependent on the successful demonstration of cyclin D1 overexpression in cases that fit clinical, morphologic, and immunophenotypical profiles. Accurate diagnosis in these cases is important due to the aggressive progression and poor survival times associated with this type of lymphoma. However, demonstration of cyclin D1 by immunohistochemistry on formalin-fixed, paraffin-embedded tissue is often fraught with technical difficulties, chiefly poor staining intensity largely due to insufficient antigen retrieval. The authors report a simple antigen retrieval technique that combines both heat and enzymatic treatment to demonstrate cyclin D1 overexpression. Furthermore, it appears that the antigen retrieval method is the key factor in the demonstration of cyclin D1, as detection systems with differing sensitivities (labeled streptavidin biotin [ABC] and dextran polymeric conjugate [Envision]) fail to demonstrate any significant differences in their staining intensities. This isa simple, cost-effective method that can be performed manually or can easily be adapted to suit automated staining systems.
Collections Ireland -> Royal College of Surgeons in Ireland -> PubMed

Full list of authors on original publication

Elaine W Kay, Mary B Leader, Anthony O'Grady, Fiona Lonergan, Robert Cummins

Experts in our system

1
Elaine W Kay
Royal College of Surgeons in Ireland
Total Publications: 157
 
2
M Leader
Royal College of Surgeons in Ireland
 
3
Anthony O'Grady
Royal College of Surgeons in Ireland
Total Publications: 35
 
4
Robert Cummins
Royal College of Surgeons in Ireland
Total Publications: 22