Type

Journal Article

Authors

E W Kay
F E Murray
M B Leader
A O'Grady
K Sheahan
C Steele
K M Sheehan

Subjects

Biochemistry

Topics
antigens cd colorectal neoplasms ulcer pathology biosynthesis prostaglandin endoperoxide synthases humans blood supply immunohistochemistry ptgs2 protein human macrophages membrane proteins metabolism antigens differentiation myelomonocytic antigens cd34 cd68 antigen human cyclooxygenase 2 neovascularization pathologic enzymology epithelium

Association between cyclooxygenase-2-expressing macrophages, ulceration and microvessel density in colorectal cancer. (2005)

Abstract In colorectal carcinomas, cyclooxygenase-2 (COX-2) is expressed predominantly by epithelial cells and is implicated in tumour progression. Tumour-associated macrophages may influence tumour growth, proliferative rate and angiogenesis and also express COX-2 when activated. Thus they may play an important stromal-epithelial role in carcinogenesis. Tauhe aim of this study was to define the relationship between microvessel density (MVD), tumour COX-2 and macrophage COX-2 expression. Sixty-five cases of formalin-fixed paraffin-embedded colorectal cancer were included in the study. Tissues were immunostained for COX-2, CD68 (macrophage marker) and CD34 (endothelial marker to assess MVD). Thirty-six cases were grossly ulcerated cancers and 29 cases showed focal/microscopic ulceration. Macrophages were in high concentration at the base of ulcerated areas, and were also diffusely dispersed within tumoral stroma. However, the pattern of macrophage COX-2 expression revealed two populations of macrophages--those deep within the tumour (negative for COX-2) and those at the base of ulcers (positive for COX-2). In all cases, the tumour epithelial cells expressed COX-2. MVD was higher at the base of ulcers, adjacent to COX-2+ macrophages, and was lower deep within the tumour. In colorectal cancers, macrophages may have a dual role. Those concentrated at the base of the ulcers, where there is an associated high MVD, may induce angiogenesis, but their function may be in a healing/repair process. The lack of COX-2+ macrophages and lower MVD deep within the tumour suggests that it may be the epithelial COX-2 component that is important in tumour progression.
Collections Ireland -> Royal College of Surgeons in Ireland -> PubMed

Full list of authors on original publication

E W Kay, F E Murray, M B Leader, A O'Grady, K Sheahan, C Steele, K M Sheehan

Experts in our system

1
Elaine W Kay
Royal College of Surgeons in Ireland
Total Publications: 157
 
2
F E Murray
Royal College of Surgeons in Ireland
 
3
M Leader
Royal College of Surgeons in Ireland
 
4
Anthony O'Grady
Royal College of Surgeons in Ireland
Total Publications: 35
 
5
Kieran Sheahan
University College Dublin
Total Publications: 40
 
6
K M Sheehan
Dublin City University
Total Publications: 14