Type

Journal Article

Authors

Patricia B Maguire
Desmond J Fitzgerald
Andrew Emili
Dolores J Cahill
James P McRedmond
Orina Belton
Thomas Kislinger
Sinead Toomey
Gerard Cagney
Judith A Coppinger

Subjects

Biochemistry

Topics
physiopathology proteins proteomics mass metabolism spectrometry cyclophilin a platelet activation calcium binding proteins arteriosclerosis blood platelets electrophoresis gel two dimensional chromogranins humans physiology matrix assisted laser calu protein mouse

Characterization of the proteins released from activated platelets leads to localization of novel platelet proteins in human atherosclerotic lesions. (2003)

Abstract Proteins secreted by activated platelets can adhere to the vessel wall and promote the development of atherosclerosis and thrombosis. Despite this biologic significance, however, the complement of proteins comprising the platelet releasate is largely unknown. Using a proteomics approach, we have identified more than 300 proteins released by human platelets following thrombin activation. Many of the proteins identified were not previously attributed to platelets, including secretogranin III, a potential monocyte chemoattractant precursor; cyclophilin A, a vascular smooth muscle cell growth factor; calumenin, an inhibitor of the vitamin K epoxide reductase-warfarin interaction, as well as proteins of unknown function that map to expressed sequence tags. Secretogranin III, cyclophilin A, and calumenin were confirmed to localize in platelets and to be released upon activation. Furthermore, while absent in normal vasculature, they were identified in human atherosclerotic lesions. Therefore, these and other proteins released from platelets may contribute to atherosclerosis and to the thrombosis that complicates the disease. Moreover, as soluble extracellular proteins, they may prove suitable as novel therapeutic targets.
Collections Ireland -> Royal College of Surgeons in Ireland -> PubMed

Full list of authors on original publication

Patricia B Maguire, Desmond J Fitzgerald, Andrew Emili, Dolores J Cahill, James P McRedmond, Orina Belton, Thomas Kislinger, Sinead Toomey, Gerard Cagney, Judith A Coppinger

Experts in our system

1
P B Maguire
University College Dublin
Total Publications: 32
 
2
D J Fitzgerald
Royal College of Surgeons in Ireland
Total Publications: 101
 
3
Orina Belton
University College Dublin
Total Publications: 22
 
4
Sinead Toomey
Royal College of Surgeons in Ireland
Total Publications: 26
 
5
Gerard Cagney
Royal College of Surgeons in Ireland
Total Publications: 54