Type

Journal Article

Authors

D J Fitzgerald
F E Murray
D Cox
K M Sheehan
J C Atherton
P A Corcoran
J F Murphy
M F Byrne

Subjects

Biochemistry

Topics
enzymology epoprostenol cyclooxygenase 1 ptgs1 protein human ptgs2 protein human isoenzymes metabolism cells cultured cyclooxygenase 2 helicobacter pylori reverse transcriptase polymerase chain reaction endothelial cells biosynthesis membrane proteins physiology blotting western humans prostaglandin endoperoxide synthases

Helicobacter pylori induces cyclooxygenase-1 and cyclooxygenase-2 expression in vascular endothelial cells. (2003)

Abstract Helicobacter pylori induces cyclooxygenase activity in the stomach, although the COX isoform and cellular source are unclear. A potential source is the vascular endothelial cell, which plays a role in regulating mucosal blood flow and inflammatory cell infiltration. We examined the effect of four strains (toxigenic and non-toxigenic) of H. pylori on COX isoform expression in vascular endothelial cells. Prostaglandin synthesis was measured by enzyme immunoassay and COX isozyme expression determined by Western blot and RT-PCR. Gene induction was examined using 5' deletion constructs of the COX-1 and COX-2 promoters coupled with luciferase. All H. pylori strains induced prostaglandin generation and expression of both COX-1 and COX-2 in HUVEC, although this was most pronounced with the highly toxigenic strain H. pylori 60190. Treatment of the cells with selective COX inhibitors demonstrated that COX-1 was predominantly responsible for the enhanced generation of prostacyclin induced by H. pylori 60190. Similar results were seen with H. pylori broth culture filtrates, suggesting that a secreted product was responsible. Induction of COX-2 reflected both enhanced gene expression and stabilization of the mRNA. H. pylori increased both COX-1 and COX-2 activity in vascular endothelial cells. This increased generation of endothelial cell prostacyclin may play a role in modulating mucosal blood flow, platelet function and inflammatory cell infiltration in response to H. pylori infection. The regulation of COX-1 at the transcriptional level by H. pylori described in this study is a novel finding and calls into question the traditional description of COX-1 as a purely constitutive, housekeeping gene.
Collections Ireland -> Royal College of Surgeons in Ireland -> PubMed

Full list of authors on original publication

D J Fitzgerald, F E Murray, D Cox, K M Sheehan, J C Atherton, P A Corcoran, J F Murphy, M F Byrne

Experts in our system

1
D J Fitzgerald
Royal College of Surgeons in Ireland
Total Publications: 101
 
2
F E Murray
Royal College of Surgeons in Ireland
 
3
Dermot Cox
Royal College of Surgeons in Ireland
Total Publications: 51
 
4
K M Sheehan
Dublin City University
Total Publications: 14
 
5
Paul Corcoran
University College Cork
Total Publications: 90