Type

Journal Article

Authors

Noel G McElvaney
Shane O'Neill
Paul B McCray
Rodney L Levine
Stephen G Smith
Catherine M Greene
Clifford C Taggart

Subjects

Microbiology

Topics
mass beta defensin 3 human growth development matrix assisted laser diffusion antagonists inhibitors defb4a protein human humans bronchoalveolar lavage fluid anti bacterial agents microbial sensitivity tests time factors spectrometry elastic tissue serine proteinase inhibitors cystic fibrosis pseudomonas aeruginosa chemistry enzymology beta defensins cathepsins microbiology

Inactivation of human beta-defensins 2 and 3 by elastolytic cathepsins. (2003)

Abstract beta-Defensins are antimicrobial peptides that contribute to the innate immune responses of eukaryotes. At least three defensins, human beta-defensins 1, 2, and 3 (HBD-1, -2, and -3), are produced by epithelial cells lining the respiratory tract and are active toward Gram-positive (HBD-3) and Gram-negative (HBD-1, -2, and -3) bacteria. It has been postulated that the antimicrobial activity of defensins is compromised by changes in airway surface liquid composition in lungs of patients with cystic fibrosis (CF), therefore contributing to the bacterial colonization of the lung by Pseudomonas and other bacteria in CF. In this report we demonstrate that HBD-2 and HBD-3 are susceptible to degradation and inactivation by the cysteine proteases cathepsins B, L, and S. In addition, we show that all three cathepsins are present and active in CF bronchoalveolar lavage. Incubation of HBD-2 and -3 with CF bronchoalveolar lavage leads to their degradation, which can be completely (HBD-2) or partially (HBD-3) inhibited by a cathepsin inhibitor. These results suggest that beta-defensins are susceptible to degradation and inactivation by host proteases, which may be important in the regulation of beta-defensin activity. In chronic lung diseases associated with infection, overexpression of cathepsins may lead to increased degradation of HBD-2 and -3, thereby favoring bacterial infection and colonization.
Collections Ireland -> Royal College of Surgeons in Ireland -> PubMed

Full list of authors on original publication

Noel G McElvaney, Shane O'Neill, Paul B McCray, Rodney L Levine, Stephen G Smith, Catherine M Greene, Clifford C Taggart

Experts in our system

1
Noel G McElvaney
Royal College of Surgeons in Ireland
Total Publications: 194
 
2
Shane J O'Neill
Royal College of Surgeons in Ireland
Total Publications: 84
 
3
Stephen Smith
Trinity College Dublin
Total Publications: 27
 
4
Catherine M Greene
Royal College of Surgeons in Ireland
Total Publications: 150
 
5
Clifford C Taggart
Royal College of Surgeons in Ireland
Total Publications: 54