Type

Journal Article

Authors

Noel G McElvaney
Shane O'Neill
Catherine M Greene
Clifford C Taggart
Siobhan Griffin

Subjects

Biochemistry

Topics
cytology up regulation polymerase chain reaction defb4a protein human bronchi physiology metabolism rna messenger humans dna primers base sequence epithelial cells beta defensins genetics leukocyte elastase

Neutrophil elastase up-regulates human beta-defensin-2 expression in human bronchial epithelial cells. (2003)

Abstract Human beta-defensin-2 (HBD-2) gene expression is induced by tumour necrosis factor-alpha, interleukin-1beta and lipopolysaccharide. The objective of this study was to investigate the effect of neutrophil elastase (NE), a major pro-inflammatory protease, on HBD-2 expression. HBD-2 gene expression was assessed by reverse transcription polymerase chain reaction in the human bronchial epithelial cell line 16HBE14o- and primary normal human bronchial epithelial (NHBE) cells. Optimal HBD-2 expression was induced with 100 nM NE. Using a HBD-2-luciferase reporter construct, luciferase activity increased significantly in 16HBE14o- cells following incubation with NE. An increase in HBD-2 protein expression was observed in primary NHBE cells after incubation with NE as assessed by laser scanning cytometry. In conclusion, NE up-regulates HBD-2 expression in bronchial epithelial cells.
Collections Ireland -> Royal College of Surgeons in Ireland -> PubMed

Full list of authors on original publication

Noel G McElvaney, Shane O'Neill, Catherine M Greene, Clifford C Taggart, Siobhan Griffin

Experts in our system

1
Noel G McElvaney
Royal College of Surgeons in Ireland
Total Publications: 194
 
2
Shane J O'Neill
Royal College of Surgeons in Ireland
Total Publications: 84
 
3
Catherine M Greene
Royal College of Surgeons in Ireland
Total Publications: 150
 
4
Clifford C Taggart
Royal College of Surgeons in Ireland
Total Publications: 54