Type

Journal Article

Authors

Dermot M Cox
Desmond J Fitzgerald
Frank E Murray
John C Atherton
Paul A Corcoran
Steven W Kerrigan
Michael F Byrne

Subjects

Microbiology

Topics
platelet glycoprotein gpib ix complex complications animals pathogenicity cho cells peptic ulcer helicobacter pylori etiology von willebrand factor antibodies bacterial physiology platelet aggregation helicobacter infections humans cricetinae

Helicobacter pylori binds von Willebrand factor and interacts with GPIb to induce platelet aggregation. (2003)

Abstract Clinical studies have suggested an association between cardiovascular disease and infection with Helicobacter pylori. We examined the effect of H. pylori on platelets and the mechanism of the interaction. Three of 5 strains of H. pylori induced platelet aggregation with a lag time of 5 +/- 2 minutes that was independent of the toxigenic genes cagA and vacA. Aggregation was inhibited completely by aspirin and a glycoprotein (GP) IIb/IIIa antagonist. Aggregation also was inhibited by monoclonal antibodies that prevented the von Willebrand factor (vWF) interaction with GPIb. vWF-coated H. pylori bound to cells transfected with GPIbalpha but not to mock transfected cells and this was inhibited by an antibody to GPIb. The interaction with platelets appeared to be mediated by vWF because platelet aggregation was blocked by an antibody to vWF. Moreover, a strain of H. pylori that induced platelet aggregation bound vWF to a greater extent than a nonaggregating strain. Aggregation also required IgG and could be inhibited by an antibody to the platelet IgG receptor (FcgammaRIIA). Some strains of H. pylori induce platelet activation mediated by H. pylori-bound vWF interacting with GPIb, and supported by IgG. These platelet-H. pylori interactions may contribute to the pathogenesis of H. pylori-associated peptic ulcer disease and to the association between H. pylori infection and cardiovascular disease, whereas local platelet effects may contribute to the pathogenesis of H. pylori-associated peptic ulcer disease.
Collections Ireland -> Royal College of Surgeons in Ireland -> PubMed

Full list of authors on original publication

Dermot M Cox, Desmond J Fitzgerald, Frank E Murray, John C Atherton, Paul A Corcoran, Steven W Kerrigan, Michael F Byrne

Experts in our system

1
Dermot Cox
Royal College of Surgeons in Ireland
Total Publications: 51
 
2
D J Fitzgerald
Royal College of Surgeons in Ireland
Total Publications: 101
 
3
F E Murray
Royal College of Surgeons in Ireland
 
4
Paul Corcoran
University College Cork
Total Publications: 90
 
5
Steven W Kerrigan
Royal College of Surgeons in Ireland
Total Publications: 37