Type

Journal Article

Authors

S J O'Neill
N G McElvaney
C Greene
C Taggart
R J Coakley

Subjects

Pharmacology

Topics
metabolism oxidants humans carbonic anhydrases physiology pharmacology chlorides carbon dioxide neutrophils acetazolamide hydrogen ion concentration bicarbonates cells cultured interleukin 8 biosynthesis

Ambient pCO2 modulates intracellular pH, intracellular oxidant generation, and interleukin-8 secretion in human neutrophils. (2002)

Abstract Although neutrophils are a critical component of the inflammatory process, their functional regulation is incompletely understood. Of note, although pCO2 varies physiologically and pathologically in the neutrophilic milieu, its affect on neutrophil biological processes is unresolved. We demonstrate here that neutrophils respond to hypo- and hypercarbia, (0.04% and 10%) by increasing and decreasing, respectively, intracellular oxidant production (basally and in response to opsonized Escherichia coli and phorbol esters). Further, hypo- and hypercarbia increase and decrease, respectively, the release of IL-8 from LPS-stimulated cells; both effects are attenuated by the carbonic anhydrase inhibitor, acetazolamide. Anion exchange did not restore pH(i) under hypocarbic conditions, however partial restoration of pH(i) under hypercarbic conditions was achieved by Na+/H+ exchange and vacuolar ATPases. Abrogation of pCO2-induced changes in pH(i) prevented hypocarbia-induced generation of reactive oxidant species. These observations suggest that CO2 modifies neutrophil activity significantly by altering pH(i).
Collections Ireland -> Royal College of Surgeons in Ireland -> PubMed

Full list of authors on original publication

S J O'Neill, N G McElvaney, C Greene, C Taggart, R J Coakley

Experts in our system

1
Shane J O'Neill
Royal College of Surgeons in Ireland
Total Publications: 84
 
2
Noel G McElvaney
Royal College of Surgeons in Ireland
Total Publications: 194
 
3
Catherine M Greene
Royal College of Surgeons in Ireland
Total Publications: 150
 
4
Clifford C Taggart
Royal College of Surgeons in Ireland
Total Publications: 54