Journal Article


N G McElvaney
S J O'Neill
G Canny
P Greally
R J Coakley
C Taggart



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Increased elastase release by CF neutrophils is mediated by tumor necrosis factor-alpha and interleukin-8. (2000)

Abstract Cystic fibrosis (CF) is a lethal, hereditary disorder characterized by a neutrophil-dominated inflammation of the lung. We sought to determine whether neutrophils from individuals with CF release more neutrophil elastase (NE) than neutrophils from normal subjects. Our results showed that peripheral blood neutrophils (PBNs) from normal subjects and individuals with CF contained similar amounts of NE, but after preincubation with CF bronchoalveolar lavage (BAL) fluid, significantly more NE was released by CF PBNs, a release that was amplified further by incubation with opsonized Escherichia coli. To determine which components of CF BAL fluid stimulated this excessive NE release from CF PBNs, we repeated the experiments after neutralization or immunoprecipitation of tumor necrosis factor (TNF)-alpha and interleukin (IL)-8 in CF BAL fluid. We found that subsequent NE release from CF PBNs was reduced significantly when TNF-alpha and IL-8 were removed from CF BAL fluid. When TNF-alpha and IL-8 were used as activating stimuli, CF PBNs released significantly greater amounts of NE compared with PBNs from control subjects and individuals with bronchiectasis. These results indicate that CF PBNs respond abnormally to TNF-alpha and IL-8 in CF BAL fluid and react to opsonized bacteria by releasing more NE. This may help explain the increased NE burden seen in this condition.
Collections Ireland -> Royal College of Surgeons in Ireland -> PubMed

Full list of authors on original publication

N G McElvaney, S J O'Neill, G Canny, P Greally, R J Coakley, C Taggart

Experts in our system

Noel G McElvaney
Royal College of Surgeons in Ireland
Total Publications: 194
Shane J O'Neill
Royal College of Surgeons in Ireland
Total Publications: 84
Peter Greally
TU Dublin (Tallaght Campus)
Total Publications: 30
Clifford C Taggart
Royal College of Surgeons in Ireland
Total Publications: 54