Type

Journal Article

Authors

M T Kelly
R O'Kennedy
G P McMahon

Subjects

Microbiology

Topics
sensitivity and specificity blood humans chromatography high pressure liquid reproducibility of results taurine spectrophotometry ultraviolet

High-performance liquid chromatographic determination of taurine in human plasma using pre-column extraction and derivatization. (1996)

Abstract Plasma samples (100 microliters) were treated with 150 microliters of acetonitrile and centrifuged at 5800 g for 10 min and 50 microliters of 10 mM borate buffer (pH 9.2) were added to the supernatant solution. This was followed by the addition of a 50 microliters aliquot of 5 mM fluorescamine in acetonitrile and immediate vortex mixing. A 20 microliters sample was injected on to a reversed-phase HPLC system using a Bondclone C-18 10 microns analytical column (300 mm x 3.9 mm). The mobile phase was tetrahydrofuran-acetonitrile-phosphate buffer (15 mM, pH 3.5) (4:24:72, v/v/v). The taurine derivative was detected by measuring the UV absorbance of 385 nm. Platelet-poor plasma samples were spiked with known amounts of taurine and inter- and intra-assay calibration curves were obtained. The method was applied to the determination of taurine in platelet-rich plasma.
Collections Ireland -> Royal College of Surgeons in Ireland -> PubMed

Full list of authors on original publication

M T Kelly, R O'Kennedy, G P McMahon

Experts in our system

1
M T Kelly
Dublin City University
Total Publications: 21
 
2
R O'Kennedy
Dublin City University
Total Publications: 197