Type

Journal Article

Authors

Paul D. Cotter
R Paul Ross
Colin Hill
Paula M. O'Connor
Lorraine A Draper
Clare Piper
Caitriona M Guinane

Subjects

Agriculture & Food Science

Topics
gastrointestinal tract git regulation environmental conditions green fluorescent protein promoter red fluorescent protein bacteriocins lactobacillus salivarius environmental factors promoter activity environmental impact

Impact of environmental factors on bacteriocin promoter activity in gut-derived Lactobacillus salivarius. (2015)

Abstract Bacteriocin production is regarded as a desirable probiotic trait that helps colonisation and persistence in the gastrointestinal tract (GIT). Strains of Lactobacillus salivarius, a species associated with the GIT, are regarded as promising probiotic candidates and have a number of associated bacteriocins documented to date. Among these include multiple class IIb bacteriocins (salivaricin T, salivaricin P and ABP-118) and the class IId bacteriocin bactofencin A, that show activity against medically important pathogens. However, the production of a bacteriocin in laboratory media does not assure production under stressful environmental conditions, such as those encountered within the GIT. To allow this issue to be addressed, the promoter regions located upstream of the structural genes encoding the aforementioned L. salivarius bacteriocins were fused to a number of reporter proteins (green fluorescent protein [GFP], red fluorescent protein [RFP] and luciferase [Lux]). Of these only transcriptional fusions to GFP generated signals of sufficient strength to study promoter activity in L. salivarius. While analysis of the class IIb promoter regions revealed relatively weak GFP expression, assessment of the promoter of the anti-staphylococcal bacteriocin bactofencin A indicated a strong promoter that is most active in the absence of the antimicrobial peptide and positively induced in the presence of mild environmental stresses, including simulated gastric fluid. Taken together this data provides information on factors that influence bacteriocin production, which will assist in developing strategies to optimise the in vivo and in vitro production of these antimicrobials.
Collections Ireland -> Teagasc -> PubMed
Ireland -> Teagasc -> Food Biosciences
Ireland -> Teagasc -> Food Biosciences
Ireland -> Teagasc -> Food Programme
Ireland -> Teagasc -> Food Programme

Full list of authors on original publication

Paul D. Cotter, R Paul Ross, Colin Hill, Paula M. O'Connor, Lorraine A Draper, Clare Piper, Caitriona M Guinane

Experts in our system

1
Paul D. Cotter
Teagasc
Total Publications: 253
 
2
R Paul Ross
Teagasc
Total Publications: 441
 
3
Colin Hill
University College Cork
Total Publications: 351
 
4
Paula M. O'Connor
Teagasc
Total Publications: 85
 
5
Lorraine A Draper
University College Cork
Total Publications: 24
 
6
Caitriona M Guinane
Teagasc
Total Publications: 35