Journal Article


Kevin O'Connor
Padraig O'Kiely
Ramesh P Babu
Maria Tuohy
Vijai Kumar Gupta
Anthonia O'Donovan
Trevor Woods
Shane T Kenny
Reeta Davis
Federico Cerrone


Agriculture & Food Science

carbon chain length grass source press production cell density molecular weight

Use of a mannitol rich ensiled grass press juice (EGPJ) as a sole carbon source for polyhydroxyalkanoates (PHAs) production through high cell density cultivation. (2015)

Abstract This study demonstrates the use of a mannitol rich ensiled grass press juice (EGPJ) as a renewable carbon substrate for polyhydroxyalkanoates (PHA) production in shaking flask experiments and fed-batch stirred tank reactor cultivations. Fed-batch cultivations of Burkholderia sacchari IPT101 using EGPJ as sole carbon source produced 44.5 g/L CDW containing 33% polyhydroxybutyrate (PHB) in 36 h, while Pseudomonas chlororaphis IMD555 produced a CDW of 37 g/L containing 10% of medium chain length polyhydroxyalkanoates (mcl-PHA) in 34 h. PHB and mcl-PHA extracted from B. sacchari IPT101 and P. chlororaphis IMD555, grown on EGPJ, had a molecular weight of 548 kg/mol and 115.4 kg/mol, respectively. While mcl-PHA can be produced from EGPJ, PHB production is more interesting as there is a 4-fold higher volumetric productivity compared to mcl-PHA.
Collections Ireland -> Teagasc -> PubMed

Full list of authors on original publication

Kevin O'Connor, Padraig O'Kiely, Ramesh P Babu, Maria Tuohy, Vijai Kumar Gupta, Anthonia O'Donovan, Trevor Woods, Shane T Kenny, Reeta Davis, Federico Cerrone

Experts in our system

Kevin E O'Connor
University College Dublin
Total Publications: 52
Padraig O'Kiely
Total Publications: 71
Vijai Kumar Gupta
National University of Ireland Galway
Trevor Woods
Trinity College Dublin
Total Publications: 11
Shane T Kenny
University College Dublin
Total Publications: 15