Journal Article


Torres Sweeney
Amanda Lohan
Bryan Markey
Jai Mehta
Bojlul Bahar
Orla M Keane
John Browne
Paul McGettigan
James P Hanrahan
Barbara Good
and 1 others



network analysis transcriptome receptors pattern recognition variation resistance rna seq differentially expressed genes lymph nodes

Variation in the Ovine Abomasal Lymph Node Transcriptome between Breeds Known to Differ in Resistance to the Gastrointestinal Nematode. (2014)

Abstract Texel lambs are known to be more resistant to gastrointestinal nematode (GIN) infection than Suffolk lambs, with a greater ability to limit infection. The objectives of this study were to: 1) profile the whole transcriptome of abomasal lymph node tissue of GIN-free Texel and Suffolk lambs; 2) identify differentially expressed genes and characterize the immune-related biological pathways and networks associated with these genes. Abomasal lymph nodes were collected from Texel (n = 6) and Suffolk (n = 4) lambs aged 19 weeks that had been GIN-free since 6 weeks of age. Whole transcriptome profiling was performed using RNA-seq on the Illumina platform. At the time of conducting this study, a well annotated Ovine genome was not available and hence the sequence reads were aligned with the Bovine (UMD3.1) genome. Identification of differentially expressed genes was followed by pathway and network analysis. The Suffolk breed accounted for significantly more of the differentially expressed genes, (276 more highly expressed in Suffolk v 162 in Texel; P < 0.001). The four most significant differentially expressed pathways were all related to immunity and were classified as: Role of Pattern Recognition Receptors in Recognition of Bacteria and Viruses, Activation of IRF by Cytosolic Pattern Recognition Receptors, Role of RIG-I-like Receptors in Antiviral Innate Immunity, and Interferon Signaling. Of significance is the fact that all of these four pathways were more highly expressed in the Suffolk. These data suggest that in a GIN-free environment, Suffolk lambs have a more active immune profile relative to the Texel: this immune profile may contribute to the poorer efficiency of response to a GIN challenge in the Suffolk breed compared to the Texel breed.
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Full list of authors on original publication

Torres Sweeney, Amanda Lohan, Bryan Markey, Jai Mehta, Bojlul Bahar, Orla M Keane, John Browne, Paul McGettigan, James P Hanrahan, Barbara Good and 1 others

Experts in our system

T Sweeney
University College Dublin
Total Publications: 138
B K Markey
University College Dublin
Total Publications: 43
B Bahar
University College Dublin
Total Publications: 39
Orla M Keane
Total Publications: 20
John A Browne
University College Dublin
Total Publications: 73
Paul A. McGettigan
University College Dublin
Total Publications: 46
J P Hanrahan
Total Publications: 59
Barbara Good
Total Publications: 36